详细说明
- Purity>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED 50 for this effect is 60-360 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # ).
- SourceMouse myeloma cell line, NS0-derived Thr25-Arg302, with a C-terminal 6-His tag
- Accession #
- N-terminal Sequence
AnalysisThr25 - Structure / FormDisulfide-linked homodimer
- Predicted Molecular Mass31 kDa
- SDS-PAGE34-43 kDa, reducing conditions
9405-SO | | |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Bioactivity | Recombinant Human Stanniocalcin 2/STC-2 (Catalog # 9405-SO) inhibits Recombinant Mouse Wnt-3a induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 60-360 ng/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt‑3a (Catalog # ). |
- References:
- Luo, C. et al. (2005). Endocrinology 146:469.
- Tagliabracci, V. S. et al. (2015). Cell 161:1619.
- Ishibashi, K. et al. (1998). Biochem. Biophys. Res. Commun. 250:252.
- Kim PH, et al. (2015) BMB Rep. 48:702.
- Wang Y, et al. (2015) Biochem Biophys Res Commun. 466:362.
- Hou J, et al. (2015) PLoS One. 10:e0122179.
- Zhou J, et al. (2016) Mol Med Rep. 14:5653.
- Wu S, et al. (2006) J Biol Chem. 281: 5120.
- Shen XJ, et al. (2014) Int J Clin Exp Pathol. 7: 8770.
- Na SS, et al. (2015) Biochim Biophys Acta. 1854:668.
- Entrez Gene IDs:8614 (Human); 20856 (Mouse)
- Alternate Names:Stanniocalcin 2; stanniocalcin-2; STC2; STC-2; STC-2Stanniocalcin-related protein; STC-related protein; STCRP