Kit Summary

For the differentiation of Th1 cells from a preparation of CD4⁺ T cells isolated from mouse splenocytes.

Key Benefits

• Provides optimized reagents needed to induce Th1 differentiation

• Yields ~80 x 10⁷ CD4⁺ T cells, of which 70-90% are IFN-γ⁺ Th1 polarized

• Contains high quality bioactive proteins

• Includes straightforward procedures

• Does not require specialized instrumentation

Why Expand and Differentiate Mouse Th1 and Th2 Cells Ex Vivo?

T helper type 1 (Th1) cells are a lineage of CD4⁺ effector T cells that promote cell-mediated immune responses against intracellular viral and bacterial pathogens. Enhanced Th1 responses are associated with autoimmune diseases including rheumatoid arthritis, multiple sclerosis, inflammatory bowel disease, and type-1 diabetes. Differentiation of CD4⁺ effector T cells into the Th1 lineage is promoted by cytokines such as IL-12 and IFN-gamma. Th1 cells secrete IFN-gamma, IL-10, and TNF-alpha. The CellXVivo™ Mouse Th1 Cell Differentiation Kit contains optimized reagents for Th1 differentiation from naïve CD4⁺ cells.

Kit Contents

This kit contains the following reagents for the ex vivo differentiation of mouse Th1 cells.

• Hamster Anti-Mouse CD3, Th1

• Mouse Th1 Reagent 1

• Mouse Th1 Reagent 2

• Mouse Th1 Reagent 3

• Mouse Th1 Reagent 4

• Reconstitution Buffer 1

• Reconstitution Buffer 2

• 20X Wash Buffer

The quantity of components in this kit is sufficient to differentiate approximately 8 x 10⁶ naïve CD4⁺ T cells, and generate 8 x 10⁷ CD4⁺ cells of which 70-90% are IFN-gamma⁺ Th1 polarized cells.

Note: Results may vary due to strain, age, and/or the health of the mice used for isolation.

Stability and Storage

Store the unopened kit at ≤-20 °C. Do not use past the kit expiration date.

Limitations

• FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

• The safety and efficacy of this product in diagnostic or other clinical uses has not been established.

• This reagent should not be used beyond the expiration date indicated on the label.

• Results may vary due to variations among cells derived from different donors.

Data Examples

Preparation and Storage

• Stability & Storage

  Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.