• Purity

  >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Human SLITRK5 (Catalog #  is coated at 500 ng/mL, Recombinant Human PSG-1 (Catalog # 9) binds with an ED    ₅₀ = 50-300 ng/mL.

• Source

  Mouse myeloma cell line, NS0-derived Gln35-Pro419, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Gln35 & Val36

• Predicted Molecular Mass

  44 kDa

• SDS-PAGE

  64-77 kDa, reducing conditions

Data Images

Background: PSG-1

Pregnancy-specific beta-1-glycoprotein 1 (PSG-1), also called SP1, PSG1 or B1G1 and designated CD66f, is a secreted glycoprotein of the human PSG family within the CEA (carcinoembryonic antigen) superfamily (1-3). PSG-1 shares 84-91% amino acid (aa) sequence identity with the most similar family members, PSG-3, -4, -6, -7, and -8. Mature PSG-1 contains a V-type Ig-like domain that is important for adhesion (N1), and three C2-type Ig-like domains (A1, A2 and B2). Three potential isoforms vary at the extreme C-terminus. Although rodents express pregnancy-specific glycoproteins, identity with human PSG proteins is low and limited to the N1 domain (3). PSG1 is one of the most abundant fetal proteins found in maternal blood during pregnancy. It is secreted by syncytiotrophoblasts and exerts proangiogenic effect by inducing VEGF-A secretion and formation of tubes in endothelial cells (4). PSG-1 also serves an immunomodulatory function through  
up-regulation of TGF-beta in macrophages, monocytes, and trophoblasts (5). In addition PSG-1 has been shown to induce secretion of anti-inflammatory cytokines  
IL-10 and IL-6 in human monocytes (6). PSG-1 binds Integrin alpha IIb beta 3 and inhibits platelet-fibrinogen interactions (7). Recent studies also suggest PSG-1's involvement in cancer biology and correlation with chemotherapy resistance in breast cancer (8).

• References:

1. Watanabe, S. and J.Y. Chou (1988) J. Biol. Chem. 263:2049.

2. Leslie, K.K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5822.

3. McLellan, A.S. et al. (2005) BMC Evol. Biol. 5:39.

4. Lisboa, F. et al. (2011) J Biol Chem. 286:7577.

5. Ha, C. et al. (2010) Biol Rep. 83:27.

6. Snyder, S. et al. (2001) Am J Reprod Immunol. 45:205.

7. Shanley D. et al. (2013) PLoS One. 8:e57491.

8. He, D. et al. (2016) Clin Sci (Lond). 130:2267.

• Long Name:

  Pregnancy-specific beta-1-glycoprotein 1

• Entrez Gene IDs:

  5669 (Human)

• Alternate Names:

  B1G1; B1G1CD66 antigen-like family member F; CD66f antigen; CD66f; DHFRP2; Fetal liver non-specific cross-reactive antigen 1/2; FLJ90598; FLJ90654; FL-NCA-1/2; PBG1; pregnancy specific beta-1-glycoprotein 1; pregnancy-specific B-1 glycoprotein; Pregnancy-specific beta-1 glycoprotein C/D; pregnancy-specific beta-1-glycoprotein 1; Pregnancy-specific glycoprotein 1; PS-beta-C/D; PS-beta-G-1; PSbG1; PSBG-1; PSBG1SP1; PSG1; PSG-1; PSG95; PSGGA; PSGGAPSG95; PSGIIA