详细说明
- Purity>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its binding ability in a functional ELISA. When Recombinant Human SLITRK5 (Catalog # is coated at 500 ng/mL, Recombinant Human PSG-1 (Catalog # 9) binds with an ED 50 = 50-300 ng/mL.
- SourceMouse myeloma cell line, NS0-derived Gln35-Pro419, with a C-terminal 6-His tag
- Accession #
- N-terminal Sequence
AnalysisGln35 & Val36 - Predicted Molecular Mass44 kDa
- SDS-PAGE64-77 kDa, reducing conditions
9334-P1 | | |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with trehalose. | ||
Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Bioactivity | WhenRecombinant Human SLITRK5 (Catalog # ) is coated at 500 ng/mL,Recombinant Human PSG-1 (Catalog # 9334-P1) binds with an ED50 = 50-300 ng/mL. |
up-regulation of TGF-beta in macrophages, monocytes, and trophoblasts (5). In addition PSG-1 has been shown to induce secretion of anti-inflammatory cytokines
IL-10 and IL-6 in human monocytes (6). PSG-1 binds Integrin alpha IIb beta 3 and inhibits platelet-fibrinogen interactions (7). Recent studies also suggest PSG-1's involvement in cancer biology and correlation with chemotherapy resistance in breast cancer (8).
- References:
- Watanabe, S. and J.Y. Chou (1988) J. Biol. Chem. 263:2049.
- Leslie, K.K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5822.
- McLellan, A.S. et al. (2005) BMC Evol. Biol. 5:39.
- Lisboa, F. et al. (2011) J Biol Chem. 286:7577.
- Ha, C. et al. (2010) Biol Rep. 83:27.
- Snyder, S. et al. (2001) Am J Reprod Immunol. 45:205.
- Shanley D. et al. (2013) PLoS One. 8:e57491.
- He, D. et al. (2016) Clin Sci (Lond). 130:2267.
- Long Name:Pregnancy-specific beta-1-glycoprotein 1
- Entrez Gene IDs:5669 (Human)
- Alternate Names:B1G1; B1G1CD66 antigen-like family member F; CD66f antigen; CD66f; DHFRP2; Fetal liver non-specific cross-reactive antigen 1/2; FLJ90598; FLJ90654; FL-NCA-1/2; PBG1; pregnancy specific beta-1-glycoprotein 1; pregnancy-specific B-1 glycoprotein; Pregnancy-specific beta-1 glycoprotein C/D; pregnancy-specific beta-1-glycoprotein 1; Pregnancy-specific glycoprotein 1; PS-beta-C/D; PS-beta-G-1; PSbG1; PSBG-1; PSBG1SP1; PSG1; PSG-1; PSG95; PSGGA; PSGGAPSG95; PSGIIA