详细说明
- Purity>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its binding ability in a functional ELISA. When Recombinant Human Podocin/NPHS2 (Catalog # ) is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human Nephrin that produces 50% of the optimal binding response is 0.3-1.5 μg/mL.
- SourceMouse myeloma cell line, NS0-derived Gln23-Thr1029, with a C-terminal 6-His tag
- Accession #
- N-terminal Sequence
AnalysisNo results obtained. Gln23 inferred from enzymatic pyroglutamate treatment revealing Leu24. - Predicted Molecular Mass110 kDa
- SDS-PAGE119-155 kDa, reducing conditions
9399-NN | | |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. | ||
Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Bioactivity | When Recombinant Human Podocin/NPHS2 (Catalog # ) is coatedonto a microplate at 2 μg/mL, Recombinant Human Nephrin (Catalog # 9399-NN) bindswith an ED50 of 0.3-1.5 µg/mL. |
- References:
- Ruotsalainen, V. et al. 1999, Proc. Natl. Acad. Sci. USA 96: 7962.
- Holzman, L.B. et al. 1999, Kidney Int. 56:1481.
- Putaala, H. et al. 2000, J. Am. Soc. Nephrol. 11:991.
- Beltcheva, O. et al. 2003, J. Am. Soc. Nephrol. 14:352.
- Putaala, H. et al. 2001, Hum. Mol. Genet. 10:1.
- Khoshnoodi, J. et al. 2003, Am. J. Pathol. 163:2337.
- Ruotsalainen, V. et al. 2000, Am. J. Pathol. 157:1905.
- Barletta, G.M. et al. 2003, J. Biol. Chem. 278:19266.
- Huber, T.B. et al. 2001, J. Biol. Chem. 276:41543.
- Lehtonen, S. et al. 2004, Am. J. Pathol. 165:923.
- Lehtonen, S. et al. 2005, Proc. Natl. Acad. Sci. 102:9814.
- Verma, R. et al. 2006, J. Clin. Invest. 116:1346.
- Foster, R.R. et al. 2005, Am. J. Physiol. Renal Physiol. 288:F48.
- Coward, R.J. et al. 2007, Diabetes 56:1127.
- Cooper, M.E. et al. 2002, Semin. Nephrol. 22:393.
- Kestila¨, M. et al. 1998, Mol. Cell. 1:575.
- Entrez Gene IDs:4868 (Human); 54631 (Mouse); 64563 (Rat)
- Alternate Names:CNF; Nephrin; nephrosis 1, congenital, Finnish type (nephrin); NPHNCNF; NPHS1; Renal glomerulus-specific cell adhesion receptor