详细说明
- Purity>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED 50 for this effect is 0.3-1.5 μg/mL.
- SourceMouse myeloma cell line, NS0-derived
Mouse AMIGO3
(Thr20-Pro378)
Accession # Q8C2S7IEGRMDP Mouse IgG2a
(Glu98-Lys330)N-terminus C-terminus - Accession #
- N-terminal Sequence
AnalysisThr20 - Structure / FormDisulfide-linked homodimer
- Predicted Molecular Mass67 kDa
- SDS-PAGE78-91 kDa, reducing conditions
9425-AM | | |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 200 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Bioactivity | Recombinant Mouse AMIGO3 Fc Chimera (Catalog #9425‑AM) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3antibody. The ED50 for this effect is 0.3-1.5 μg/mL. |
- References:
- Kuja-Panula J, et al. (2003). J Cell Biol 160:963.
- Ahmed Z, et al. (2013) PLoS One. 16:e61878.
- Long Name:Amphoterin-induced Gene and ORF 3
- Entrez Gene IDs:386724 (Human); 320844 (Mouse)
- Alternate Names:adhesion molecule with Ig-like domain 3; ALI3; Alivin-3; AMIGO3; AMIGO-3; amphoterin-induced gene and ORF 3; amphoterin-induced protein 3; KIAA1851; MGC120552