Human DC-SIGN/CD209 Alexa Fluor 700 MAb (Clone 120507) 100 TESTS

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Human DC-SIGN/CD209 Alexa Fluor 700 MAb (Clone 120507) 100 TESTS信息二维码

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产品介绍

    基本参数

    详细说明

    • Species Reactivity
      Human
    • Specificity
      Detects human DC‑SIGN/CD209 on transfected NIH/3T3 cells and on monocyte derived dendritic cells. Does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.
    • Source
      Monoclonal Mouse IgG 2B Clone # 120507
    • Purification
      Protein A or G purified from hybridoma culture supernatant
    • Immunogen
      NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGN/CD209
    • Formulation
      Supplied in a saline solution containing BSA and Sodium Azide.
    • Label
      Alexa Fluor 700
    Applications
    •  
      Recommended
      Concentration
      Sample
    • Flow Cytometry
      5 µL/10 6 cells
      See below
    Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
    Data Examples
    Flow Cytometry

    Detection of DC‑SIGN/CD209 in NIH‑3T3 Mouse Cell Line Transfected with Human DC-SIGN/CD209 by Flow Cytometry. NIH‑3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGN/CD209 was stained with Mouse Anti-Human DC‑SIGN/CD209 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB161N, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for .

    Preparation and Storage
    • Shipping
      The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
    • Stability & Storage
      Protect from light. Do not freeze.
      • 12 months from date of receipt, 2 to 8 °C as supplied.
    Background: DC-SIGN/CD209

    Human DC-SIGN (Dendritic Cell-Specific ICAM-3 Grabbing Non-integrin) also known as CD209 is a member of the chromosome 19 C-type lectin family that includes DC-SIGN, DC-SIGN-related protein, CD23 and LSECtin (1). DC-SIGN was initially reported to be a 46 kDa, 404 amino acid (aa) type II transmembrane protein that contained a 40 aa cytoplasmic N-terminus, a 21 aa transmembrane segment, and a 343 aa extracellular C-terminus (2). The extracellular region contains a distal, 115 aa Ca++-dependent carbohydrate-binding lectin domain and a membrane-proximal linker segment that is composed of seven 23 aa repeats (2, 3). The lectin domain is believed to preferably bind mannose, either within the context of ICAM-3 (on T cells) or ICAM-2 (on endothelial cells) (2, 4, 5). DC-SIGN expression appears to be limited to dendritic cells (DC) and macrophages (6), and DC interaction with the ICAMs both aids DC cell trafficking and immunological synapse formation (7). Since the original report on DC-SIGN, multiple splice forms have been discovered, generating both membrane-bound and soluble forms (3). There are eight type A isoforms, all of which begin with the same 15 aa of exon 1a. Four contain the transmembrane region of exon II, and four do not (i.e., are soluble). Among these eight type A isoforms, only three retain the entire 343 aa found in the full length form described in reference #2 (the full length form is referred to as Type I mDC-SIGN1A) (3). Five additional isoforms utilize an alternate start site, and these are referred to as type B isoforms. These all show a 35 aa cytoplasmic domain. One also has a transmembrane segment; four do not. Two of the five contain full, unspliced extracellular regions (3). All of this suggests enormous complexity in DC-SIGN biology. DC-SIGN is not well conserved across species. Human and mouse show little overall aa identity. In the lectin domain, however, human DC-SIGN shares 68% aa identity with mouse DC-SIGN (8). Human and rhesus monkey DC-SIGN share 91% aa identity over the entire extracellular region (8). A detailed description of the additional properties of this monoclonal antibody (MAB161) have been published (9, 10).

    • References:
      1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
      2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
      3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
      4. Su, S.V. et al. (2004) J. Biol. Chem. 279:19122.
      5. Cambi, A. et al. (2005) Cell. Microbiol. 7:481.
      6. Serrano-Gomez, D. et al. (2004) J. Immunol. 173:5635.
      7. Geijtenbeek, T.B.H. and Y. van Kooyk (2003) Curr. Top. Microbiol. Immunol. 276:32.
      8. Baribaud, F. et al. (2001) J. Virol. 75:10281.
      9. Wu, L. et al. (2002) J. Virol. 76:5905.
      10. Baribaud, F. et al. (2002) J. Virol.76:9135.
    • Long Name:
      Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1
    • Entrez Gene IDs:
      30835 (Human); 170786 (Mouse)
    • Alternate Names:
      CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1
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