• Species Reactivity

  Human

• Specificity

  Detects human IL-18 R alpha /IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL‑1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 70625

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5    
  Glu20-Arg329    
  Accession # Q13478

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-18 R alpha/IL-1 R5

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also known as IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also known as IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

• References:

1. Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.

2. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.

3. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.

• Long Name:

  Interleukin 18 Receptor alpha

• Entrez Gene IDs:

  8809 (Human); 16182 (Mouse)

• Alternate Names:

  CD218a; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra