详细说明
- Species ReactivityHuman
- SpecificityDetects human IL-18 R alpha /IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL‑1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.
- SourceMonoclonal Mouse IgG 1 Clone # 70625
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenMouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5
Glu20-Arg329
Accession # Q13478 - FormulationSupplied in a saline solution containing BSA and Sodium Azide.
- LabelAlexa Fluor 488
- Recommended
ConcentrationSample
- Flow Cytometry5 µL/10 6 cellsSee below
| Flow Cytometry | Detection of IL‑18 R alpha /IL‑1 R5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 10 ng/mL Recombinant Human IL‑2 (Catalog # ) and 5 μg/mL PHA for 48 hours were stained with Mouse Anti-Human IL‑18 R alpha /IL‑1 R5 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB840G, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageProtect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also known as IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also known as IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.
- References:
- Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
- Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
- Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
- Long Name:Interleukin 18 Receptor alpha
- Entrez Gene IDs:8809 (Human); 16182 (Mouse)
- Alternate Names:CD218a; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra
400-6226-992