详细说明
Species Reactivity
Mouse
Specificity
Detects mouse ADAM10 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) rhADAM10 is observed and no cross-reactivity with rhADAM8, recombinant mouse (rm) ADAM9, rmADAM15, rhBACE, or rhTACE is observed.
Source
Monoclonal Rat IgG 2A Clone # 139712
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ADAM10
Thr215-Glu673
Accession # O35598Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700
Applications
Recommended
ConcentrationSample
Flow Cytometry
5 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of ADAM10 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse ADAM10 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB946N, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: ADAM10
ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha ) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).
References:
Rooke, J. et al. (1996) Science 273:1227.
Pan, D. and Rubin, G.M. (1997) Cell 90:271.
Qi, H. et al. (1999) Science 283:91.
Lammich, S. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
Rosendahl, M.S. et al. (1997) J. Biol. Chem. 272:24588.
Chantry, A. et al. (1989) J. Biol. Chem. 264:21603.
Millichip, M.I. et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
Chantry, A. and Glynn, P. (1990) Biochem. J. 268:245.
Fahrenholz, F.S. et al. (2000) Ann. N.Y. Acad. Sci. 920:215.
Long Name:
A Disintegrin and Metalloprotease-like Domain 10
Entrez Gene IDs:
102 (Human); 11487 (Mouse)
Alternate Names:
a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10; AD10; ADAM 10; ADAM metallopeptidase domain 10; ADAM10; CD156c antigen; CD156c; CDw156; disintegrin and metalloproteinase domain-containing protein 10; EC 3.4.24; HsT18717; kuz; Kuzbanian protein homolog; Kuzbanian; MADM; MADMEC 3.4.24.81; Mammalian disintegrin-metalloprotease