Recombinant Mouse PD-1 Fc Chimera Protein, CF 100 UG

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Recombinant Mouse PD-1 Fc Chimera Protein, CF 100 UG信息二维码

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3-Amino-5-methoxycarbonylphenylboronic acid, pinacol ester  1 g 2,3-Dichloro-6-(trifluoromethyl)benzyl bromide  1 g 1-(4-Fluorophenyl)-5-methoxycarbonyl-2(1H)-pyridinone  1 g N,N-Diethyl-cyclohexane-1,4-diamine  1 g N-Methyl-DL-leucine hydrochloride  5 g N-(2-Chloroethyl) 3-boronobenzamide  5 g

产品介绍

    基本参数

    详细说明

    • Purity
      >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
    • Endotoxin Level
      <0.10 EU per 1 μg of the protein by the LAL method.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse PD-1 Fc Chimera at 1 µg/mL (100 µL/well) can bind Recombinant Mouse B7‑H1/PD‑L1 Fc Chimera (Catalog # ) with an ED 50 of 0.16-0.8 μg/mL.
    • Source
      Mouse myeloma cell line, NS0-derived
      Mouse PD-1
      (Leu25-Gln167)
      Accession # Q02242
      IEGRMD Human IgG1
      (Pro100-Lys330)
      N-terminus C-terminus
    • Accession #
    • N-terminal Sequence
      Analysis
      Leu25
    • Structure / Form
      Disulfide-linked homodimer
    • Predicted Molecular Mass
      43 kDa (monomer)
    • SDS-PAGE
      66 kDa, reducing conditions
    1021-PD
     
    Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
    Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
    Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
    Data Images
    Bioactivity

    When Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD) is coated at 1 μg/mL, Recombinant Mouse B7‑H1/PD‑L1 Fc Chimera (Catalog # ) binds with an ED50 of 0.16-0.8 μg/mL.
    Background: PD-1
    Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors (1). Other members of this family include CD28, CTLA-4, ICOS, and BTLA (2-5). Mature mouse PD-1 consists of a 149 amino acid (aa) extracellular region (ECD) with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The mouse PD-1 ECD shares 65% aa sequence identity with the human PD-1 ECD. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important for mediating PD-1 signaling. PD-1 acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) (6, 7). PD‑1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes (8, 9). Ligation of PD-L1 with PD-1 induces
    co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion (10). Thus, the PD-1:PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance (11). Finally, blockade of the PD-1: PD-L1 interaction by either antibodies or genetic manipulation accelerates tumor eradication and shows potential for improving cancer immunotherapy (12, 13).
    • References:
      1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
      2. Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116.
      3. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
      4. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
      5. Watanabe, N et al. (2003) Nat. Immunol. 4:670.
      6. Zhang, X. et al. (2004) Immunity 20:337.
      7. Lázár-Molnár, E. et al. (2008) Proc. Natl. Acad. Sci. USA 105:10483.
      8. Nishimura, H et al. (1996) Int. Immunol. 8:773.
      9. Keir, M.E. et al. (2008) Annu. Rev. Immunol. 26:677.
      10. Butte, M.J. et al. (2007) Immunity 27:111.
      11. Okazaki, T. et al. (2013) Nat. Immunol. 14:1212.
      12. Iwai, Y. et al. (2002) Proc. Natl. Acad. Sci. USA 99: 12293.
      13. Nogrady, B. (2014) Nature 513:S10.
    • Long Name:
      Programmed Death-1
    • Entrez Gene IDs:
      5133 (Human); 18566 (Mouse); 301626 (Rat); 486213 (Canine); 102123659 (Cynomolgus Monkey)
    • Alternate Names:
      CD279 antigen; CD279; hPD-1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2
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