详细说明
- Purity>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<1.0 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its binding ability in a functional ELISA. Immobilized rmIL-1F5, rmIL-1F6, or rmIL-1F8 at 1 µg/mL (100 µL/well) can bind
rmIL-1 Rrp2/his with a linear range of 0.078-5 µg/mL. - SourceMouse myeloma cell line, NS0-derived Asp22-Arg338 (Ala158Val), with a C-terminal 6-His tag
- Accession #
- N-terminal Sequence
AnalysisAsp22 - Predicted Molecular Mass36.8 kDa
- SDS-PAGE55-68 kDa, reducing conditions
| 2354-RP/CF | | 2354-RP |
| Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | |
| Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | |
| Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
IL-1 Rrp2, also known as IL-1 R6, is a member of the IL-1 R/TLR superfamily. Members of this superfamily have a characteristic Toll-IL-1 R (TIR) domain in their cytoplasmic region. This superfamily can be further divided into three subgroups based on extracellular region analogies. IL-1 Rrp2 belongs to Subgroup 1 which is characterized by the presence of at least one immunoglobulin-like domain (1). Mouse IL-1 Rrp2/IL-1R6 is a 70 kDa type I transmembrane glycoprotein that is synthesized as a 574 amino acid (aa) precursor. The precursor contains a 21 aa signal sequence, a 317 aa extracellular region, a 21 aa transmembrane segment, and a 215 aa cytoplasmic domain (2). The extracellular region contains three C-type Ig-like domains. Within the extracellular region, mouse IL-1 Rrp2 is 64% and 80% aa identical to human and rat IL-1 Rrp2, respectively. Expression of IL-1 Rrp2 is restricted to lung epithelium, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (3). Studies have demonstrated that the functional receptor for IL-1F6, IL-1F8 and IL-1F9 is a combination of IL-1 Rrp2 and IL-1 RAcP. This suggests that IL-1 Rrp2 ligands may signal in a similar fashion to IL-1 and IL-18. In each case, there is a binding receptor which, upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex (4).
- References:
- Dunne, A. and L.A. O’Neill, (2003) Sci. STKE Feb 25 (171):re3.
- Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
- Lovenberg, T.S. et al. (1996) J. Neuroimmunol. 70:113.
- Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
- Long Name:Interleukin 1 Receptor-like 2
- Entrez Gene IDs:8808 (Human); 107527 (Mouse); 171106 (Rat)
- Alternate Names:IL-1 R6; IL-1 Rrp2; IL1RL2; IL-1RL2; IL1Rrp2; IL-1Rrp2
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