• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When Recombinant Mouse MSP R/Ron Fc Chimera is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse MSP/MST1 (Catalog # ) that produces 50% of the optimal binding response is 1-6 ng/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse MSP R   alpha subunit Ser24-Arg310 Accession # CAA52754

  Mouse MSP R   beta subunit Gly311-Arg960 (Ile372Leu) Accession # CAA52754	DIEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus			C-terminus

  
   

• Accession #

• N-terminal Sequence    
  Analysis

  Ser24 ( alpha subunit) & Gly311 ( beta subunit)    
   

• Structure / Form

  Disulfide-linked tetramer containing two alpha subunits and two Fc-linked  beta subunits.

• Predicted Molecular Mass

  128.7 kDa

Background: MSP R/Ron

Macrophage stimulating protein receptor (MSP R), encoded by the human RON and the mouse Stk, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met (the receptor for hepatocyte growth factor) and chicken Sea. This family of receptors is synthesized as a single-chain precursor that is cleaved into a mature disulfide-linked heterodimer composed of an extracellular alpha chain and a membrane spanning beta chain with intrinsic tyrosine kinase activity. Mouse MSP R cDNA encodes a 1378 amino acid (aa) residue presursor protein with a 23 aa signal peptide, a 287 aa residue alpha chain (Ser 24 - Arg 310) and a 1068 aa residue transmembrane beta chain (Gly 311 - Thr 1378). Expression of MSP receptor is restricted to specific areas of the central and peripheral nervous systems, epithelial cells along the digestive tract, skin and lung, and in subpopulations of the mononuclear phagocyte lineage. Both the heterodimeric MSP and the free MSP beta chain have been shown to bind to MSP R. However, only the heterodimeric MSP binding can induce receptor dimerization and phosphorylation and cause biological activity.

• References:

1. Gaudino, G. et al. (1994) EMBO J. 13:3524.

2. Wang, M-H. et al. (1994) Science 266:117.

3. Wang, M-H. et al. (1997) J. Biol. Chem. 272:16999.

• Long Name:

  Macrophage Stimulating Protein Receptor

• Entrez Gene IDs:

  4486 (Human); 19882 (Mouse)

• Alternate Names:

  CD136 antigen; CD136; CDw136c-met-related tyrosine kinase; EC 2.7.10; EC 2.7.10.1; macrophage stimulating 1 receptor (c-met-related tyrosine kinase); MSP R; MSP receptor; MSPR; MST1R; p185-Ron; Protein-tyrosine kinase 8; PTK8 protein tyrosine kinase 8; PTK8; Ron; RONmacrophage-stimulating protein receptor; soluble RON variant 1; soluble RON variant 2; soluble RON variant 3; soluble RON variant 4