• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to block human IL-15-induced proliferation of CTLL‑2 mouse cytotoxic T cells. Ruchatz, H.     et al. (1998) J. Immunol.     160:5654. The ED    ₅₀ for this effect is 0.1-0.5 ng/mL in the presence of 0.1 ng/mL recombinant human IL-15.

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse IL-15 R alpha (Gly33 - Lys205) Accession # Q60819	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

  Contains a small amount (10%) of free IL-15 R alpha and Fc generated by proteolytic cleavage.

• Accession #

• N-terminal Sequence    
  Analysis

  Gly33

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  44.9 kDa (monomer)

• SDS-PAGE

  80-90 kDa, 42 kDa and 35 kDa, reducing conditions

Background: IL-15 R alpha

Interleukin 15 Receptor alpha (IL 15 R alpha ), also known as CD215, is a widely expressed 60 kDa transmembrane glycoprotein that plays an important role in the homeostasis and activation of NK cells and CD8⁺ memory T cells and participates in the development and function of many other hematopoietic cell types and non‑immune cell types (1 ‑ 3). Mature mouse IL‑15 R alpha consists of a 173 aa extracellular domain (ECD) containing one N‑linked glycosylation site, a 21 aa transmembrane segment, and a 37 aa cytoplasmic tail (4). Within the ECD, mouse IL‑15 R alpha shares 59% and 89% aa sequence identity with human and rat IL‑15 R alpha, respectively. Alternate splicing of mouse IL‑15 R alpha generates additional isoforms with an N‑terminal truncation or variable length deletions in the ECD. IL‑15 R alpha binds to Interleukin‑15 with high affinity (4). IL‑15 additionally interacts with lower affinity to a complex of IL‑2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL‑2 receptor complex (5, 6). The use of shared receptor components contributes to the overlapping biological effects of IL‑15 and IL‑2. The dominant mechanism of IL‑15 action is known as transpresentation in which IL‑15/IL‑15 R alpha complexes are expressed on the surface of one cell and interact with complexes of IL‑2 R beta / gamma c on adjacent cells (7). This enables cells to respond to IL‑15 even if they do not express IL‑15 R alpha (8 ‑ 10). IL‑15/IL‑15 R alpha complexes can transmit reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL‑15/IL‑15 R alpha expressing cells (11, 12). Shed soluble forms of IL‑15 R alpha retain the ability to bind tightly to IL‑15 and can inhibit IL‑15 bioactivity (4, 13, 14).

• References:

1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.

2. Di Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.

3. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.

4. Giri, J.G. et al. (1995) EMBO 14:3654.

5. Grabstein, K. et al. (1994) Science 264:965.

6. Giri, J. et al. (1994) EMBO J. 13:2822.

7. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.

8. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.

9. Dubois, S. et al. (2002) Immunity 17:537.

10. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.

11. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.

12. Neely, G.G. et al. (2004) J. Immunol. 172:4225.

13. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.

14. Mortier, E. et al. (2004) J. Immunol. 173:1681.

• Long Name:

  Interleukin 15 Receptor alpha

• Entrez Gene IDs:

  3601 (Human); 16169 (Mouse)

• Alternate Names:

  CD215 antigen; CD215; IL-15 R alpha; IL-15 receptor subunit alpha; IL15R alpha; IL15RA; IL-15Ra; IL-15R-alpha; interleukin 15 receptor, alpha; interleukin-15 receptor subunit alpha; MGC104179