详细说明
- Purity>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<0.01 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to antagonize alpha -MSH-induced cAMP accumulation in HEK293 human embryonic kidney cells transfected with human Melanocortin-4 Receptor. Ollmann, M.M. et al. (1997) Science 278:135. The ED 50 for this effect is typically 0.025-0.15 µg/mL in the presence of 10 ng/mL of alpha -MSH.
- SourceE. coli-derived Ser82-Thr131
- Accession #
- N-terminal Sequence
AnalysisSer82 - Predicted Molecular Mass5.7 kDa
634-AG | | |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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- References:
- Martin, N.M. et al. (2006) Peptides 27:333.
- Fan, W. et al. (1997) Nature 385:165.
- Ollmann, M.M. et al. (1997) Science 278:135.
- Arora, S. and Anubhuti (2006) Neuropeptides 40:375.
- Shutter, J.R. et al. (1997) Genes Dev. 11:593.
- Kiefer, L.L. et al. (1998) Biochemistry 37:991.
- Jackson, P.J. et al. (2002) Biochemistry 41:7565.
- Fong, T.M. et al. (1997) Biochem. Biophys. Res. Commun. 237:629.
- Breit, A. et al. (2006) J. Biol. Chem. 281:37447.
- Katsuki, A. et al. (2001) J. Clin. Endocrinol. Metab. 86:1921.
- Small, C.K. et al. (2001) Diabetes 50:248.
- Vink, T. et al. (2001) Mol. Psychiatry 6:325.
- Dardennes, R.M. et al. (2007) Psychoneuroendocrinology 32:106.
- Doghman, M. et al. (2007) Mol. Cell. Endocrinol. 265-266:108.
- Long Name:Agouti-related Protein
- Entrez Gene IDs:181 (Human); 11604 (Mouse)
- Alternate Names:agouti related protein homolog (mouse); agouti-related protein; AgRP; Agrt; AGRTMGC118963; ART; ARTagouti (mouse) related protein; ASIP2