• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to enhance LPS-induced IL-6 secretion by mouse splenocytes. The ED    ₅₀ for this effect is 0.4-2.4 µg/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse CD14 (Ala18-Pro345) Accession # Q4FJP7	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Ala18

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  61.8 kDa (monomer)

• SDS-PAGE

  85-95 kDa, reducing conditions

Background: CD14

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The mouse CD14 cDNA encodes a 366 amino acid (aa) residue precursor protein with a 15 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Mouse CD14 has five potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of mouse CD14 is approximately 65% and 82% identical to the human and rat proteins, respectively. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

• References:

1. Wright, S.D. et al. (1990) Science 249:1431.

2. Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.

3. Beutler, B. (2000) Current Opinion in Immunology 12:20.

4. Stelter, F. (2000) Chem. Immunol. 74:25.

• Entrez Gene IDs:

  929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)

• Alternate Names:

  CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein