详细说明
- Purity>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<1.0 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to inhibit the IL-3-induced proliferation of NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED 50 for this effect is 0.03‑0.15 µg/mL in the presence of 2.5 µg/mL Recombinant Mouse IL‑3 R beta Fc Chimera (Catalog # ) and 2 ng/mL of Recombinant Mouse IL‑3 (Catalog # ).
- SourceSpodoptera frugiperda, Sf 21 (baculovirus)-derived
Mouse IL-3 R alpha
(Ser17-Lys331)
Accession # P26952IEGRID Human IgG1
(Pro100-Lys330)N-terminus C-terminus - Accession #
- N-terminal Sequence
AnalysisSer17 - Structure / FormDisulfide-linked homodimer
- Predicted Molecular Mass61.0 kDa (monomer)
- SDS-PAGE70-80 kDa, reducing conditions
983-MR | | |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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- References:
- Broughton, S.E. et al. (2012) Immunol. Rev. 250:277.
- Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
- Miyajima, A. et al. (1993) Blood 82:1960.
- Hara, T. & A. Miyajima (1992) EMBO J. 11:1875.
- Korpelainen, E.I. et al. (1993) Proc. Natl. Acad. Sci. USA 90:11137.
- Ebner, S. et al. (2002) J. Immunol. 168:6199.
- Gregory, B. et al. (2003) J. Immunol. 170:5359.
- Dahl, C. et al. (2004) Allergy 59:1087.
- Grouard, G. et al. (1997) J. Exp. Med. 185:1101.
- Clark-Lewis, I. et al. (1984) J. Biol. Chem. 259:7488.
- Knepper, T.P. et al. (1992) Biochemistry 31:11651.
- Ogorochi, T. et al. (1992) Blood 79:895.
- Murphy, J.M. et al. (2004) J. Biol. Chem. 279:26500.
- Ichihara, M. et al. (1995) EMBO J. 14:939.
- Chen, J. et al. (2009) J. Biol. Chem. 284:5763.
- Mirza, S. et al. (2010) J. Biol. Chem. 285:22370.
- Dentelli, P. et al. (1999) J. Immunol. 163:2151.
- Long Name:Interleukin 3 Receptor alpha
- Entrez Gene IDs:3563 (Human); 16188 (Mouse)
- Alternate Names:CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 R alpha; IL-3 receptor subunit alpha; IL3R alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174