Recombinant Mouse IL-3 R alpha/CD123 Fc Chimera Protein, CF 100 UG

所属行业:生物试剂     有0人感兴趣

Recombinant Mouse IL-3 R alpha/CD123 Fc Chimera Protein, CF 100 UG信息二维码

微信扫码

 
基础投资电议
推荐指数
  • 区域全国
  • 留言暂无
  • 浏览0
  • 更新2024-07-25 20:42
  • 到期长期有效






更多>我还有产品
3-Amino-5-methoxycarbonylphenylboronic acid, pinacol ester  1 g 2,3-Dichloro-6-(trifluoromethyl)benzyl bromide  1 g 1-(4-Fluorophenyl)-5-methoxycarbonyl-2(1H)-pyridinone  1 g N,N-Diethyl-cyclohexane-1,4-diamine  1 g N-Methyl-DL-leucine hydrochloride  5 g N-(2-Chloroethyl) 3-boronobenzamide  5 g

产品介绍

    基本参数

    详细说明

    • Purity
      >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
    • Endotoxin Level
      <1.0 EU per 1 μg of the protein by the LAL method.
    • Activity
      Measured by its ability to inhibit the IL-3-induced proliferation of NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED 50 for this effect is 0.03‑0.15 µg/mL in the presence of 2.5 µg/mL Recombinant Mouse IL‑3 R beta Fc Chimera (Catalog # ) and 2 ng/mL of Recombinant Mouse IL‑3 (Catalog # ).
    • Source
      Spodoptera frugiperda, Sf 21 (baculovirus)-derived
      Mouse IL-3 R alpha
      (Ser17-Lys331)
      Accession # P26952
      IEGRID Human IgG1
      (Pro100-Lys330)
      N-terminus C-terminus
    • Accession #
    • N-terminal Sequence
      Analysis
      Ser17
    • Structure / Form
      Disulfide-linked homodimer
    • Predicted Molecular Mass
      61.0 kDa (monomer)
    • SDS-PAGE
      70-80 kDa, reducing conditions
    983-MR
     
    Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
    Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
    Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
    Background: IL-3 R alpha/CD123
    The IL-3 receptor chain (IL‑3 R alpha ; also SUT-1 and CD123) is a 60‑70 kDa member of the class I cytokine receptor family of proteins (1, 2, 3). Members of this class are type I transmembrane proteins that contain two fibronectin type III (FNIII) modules and a distinctive Trp‑Ser‑x‑Trp‑Ser peptide motif in the extracellular domain. This is in contrast to class II receptors that share the same fibronectin domains but lack both the WSxWS motif and analogous cysteine spacings. Mouse IL‑3 R alpha is synthesized as a 396 amino acid (aa) precursor that contains a 16 aa signal sequence, a 315 aa extracellular region, a 24 aa transmembrane segment, and a 41 aa cytoplasmic tail (3, 4). The molecule is expressed on multiple cell types, including endothelial cells (5), monocytes (6), eosinophils (7), basophils plus mast cells (8), and plasmacytoid CD4 + T cells (9). IL‑3 R alpha serves as a low affinity binding protein for 24‑28 kDa monomeric IL‑3 (1, 3, 4, 10, 11). Following binding, the IL‑3:IL‑3 R alpha complex likely interacts with a preformed signaling homodimer comprised of either 120 kDa AIC2A (= beta IL-3) or 130 kDa AIC2B (= beta c) chains. This tetramer appears to recruit one more IL‑3:IL‑3 R alpha complex, generating a fully functional hexamer (1, 3, 4, 12, 13). Signaling is mediated by receptor‑associated cytoplasmic kinases, as class I cytokine receptors do not possess intrinsic kinase activity. Notably, the IL‑3 R complex may not exist as a singularity, but actually form higher‑order complexes with FcR gamma, beta 1 integrin, and VEGF R2 (1). There are at least two IL‑3 Ra isoform variants in mouse. One is found in the AKR and A/J strains, and shows a deletion of aa 274‑283. This is not present at the cell membrane (14). The second (called SP2) shows a deletion of aa 20‑112, generating a 50‑55 kDa membrane form that binds IL‑3, but will only signal through beta IL-3 (15, 16). IL‑3’s choice of full‑length IL‑3 R alpha (SP1) or SP2 leads to different signaling outcomes, presumably due to the engagement of different binding sites on beta IL-3. The extracellular domain of mouse IL‑3 R alpha /SP1 shares only 44% and 29% aa sequence identity with rat and human IL‑3 R alpha, respectively. Nevertheless, human IL‑3 is reported to be active on the mouse receptor complex that is expressed on select cell types (17).
    • References:
      1. Broughton, S.E. et al. (2012) Immunol. Rev. 250:277.
      2. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
      3. Miyajima, A. et al. (1993) Blood 82:1960.
      4. Hara, T. & A. Miyajima (1992) EMBO J. 11:1875.
      5. Korpelainen, E.I. et al. (1993) Proc. Natl. Acad. Sci. USA 90:11137.
      6. Ebner, S. et al. (2002) J. Immunol. 168:6199.
      7. Gregory, B. et al. (2003) J. Immunol. 170:5359.
      8. Dahl, C. et al. (2004) Allergy 59:1087.
      9. Grouard, G. et al. (1997) J. Exp. Med. 185:1101.
      10. Clark-Lewis, I. et al. (1984) J. Biol. Chem. 259:7488.
      11. Knepper, T.P. et al. (1992) Biochemistry 31:11651.
      12. Ogorochi, T. et al. (1992) Blood 79:895.
      13. Murphy, J.M. et al. (2004) J. Biol. Chem. 279:26500.
      14. Ichihara, M. et al. (1995) EMBO J. 14:939.
      15. Chen, J. et al. (2009) J. Biol. Chem. 284:5763.
      16. Mirza, S. et al. (2010) J. Biol. Chem. 285:22370.
      17. Dentelli, P. et al. (1999) J. Immunol. 163:2151.
    • Long Name:
      Interleukin 3 Receptor alpha
    • Entrez Gene IDs:
      3563 (Human); 16188 (Mouse)
    • Alternate Names:
      CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 R alpha; IL-3 receptor subunit alpha; IL3R alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174
    举报收藏 0评论 0
 
热门项目排行榜快速发布信息
14-(2-碘代乙基)辛基苯 9
2高温波导测试
3格列美脲 98%+ RG  5g
44-溴-3,5-二氯三氟甲苯
5二次谐波
6远红外云试剂
生物试剂排行榜快速发布信息
1Recombinant Human LILR
2Recombinant Human CD47
3Recombinant Human FGF
4203mm具砂板闪式层析柱,
5pUCm-T 载体 5UG
65ml Protein A40 Plus亲
最新加入项目快速发布信息
1质粒大量抽提试剂盒(通
2实验试剂
3产品标题产品标题产品标
4实验试剂
53-Amino-5-methoxycarbo
6N,N-Diethyl-cyclohexan

猜你喜欢产品快速发布信息

相关栏目

还没找到您需要的生物试剂产品?立即发布您的求购意向,让生物试剂公司主动与您联系!

立即发布项目意向

免责声明

本网页所展示的有关【Recombinant Mouse IL-3 R alpha/CD123 Fc Chimera Protein, CF 100 UG_生物试剂_云试剂自营四店】的信息/图片/参数等由云试剂的会员【云试剂自营四店】提供,由云试剂会员【云试剂自营四店】自行对信息/图片/参数等的真实性、准确性和合法性负责,本平台(本网站)仅提供展示服务,请谨慎交易,因交易而产生的法律关系及法律纠纷由您自行协商解决,本平台(本网站)对此不承担任何责任。您在本网页可以浏览【Recombinant Mouse IL-3 R alpha/CD123 Fc Chimera Protein, CF 100 UG_生物试剂_云试剂自营四店】有关的信息/图片/价格等及提供【Recombinant Mouse IL-3 R alpha/CD123 Fc Chimera Protein, CF 100 UG_生物试剂_云试剂自营四店】的商家公司简介、联系方式等信息。

联系方式

在您的合法权益受到侵害时,欢迎您向www@yunshiji.cn邮箱发送邮件,或者进入《网站意见反馈》了解投诉处理流程,我们将竭诚为您服务,感谢您对云试剂的关注与支持!

按排行字母分类找加盟项目:

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z