详细说明
- Purity>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED 50 for this effect is 5-20 ng/mL.
- SourceE. coli-derived Ser94-Lys191
- Accession #
- N-terminal Sequence
AnalysisSer94 - Predicted Molecular Mass11.3 kDa
989-AR/CF | | 989-AR |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | |
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Amphiregulin (AR), also known as Schwannoma-derived growth factor (SDGF), is a member of the epidermal growth factor (EGF) family of growth factors which includes, AR, EGF, transforming growth factor-a (TGF-a), heparin binding EGF-like growth factor (HB-EGF), betacellulin (BTC), epiregulin, and the neuregulins-1 through -4. All EGF family members are synthesized as type I transmembrane precursors and contain one or several EGF domains in their extracellular region. The bioactive form of the proteins is released by proteolytic cleavage. The ErbB family of receptors that includes ErbB1 - B4, mediates the biological activities of the EGF family ligands. AR was originally isolated from the conditioned media of PMA-treated MCF-7 human breast carcinoma cell line. AR mRNA expression can be detected in numerous carcinoma cell lines and in the epithelial cells of various human tissues including colon, stomach, breast, ovary and kidney. AR stimulates the proliferation of keratinocytes, mammary epithelial cells, fibroblasts, astrocytes and glial cells. AR is also a growth inhibitor for certain tumor cells. The gene for AR has been mapped to human chromosome 4q13-q21 and mouse chromosome 5. Human and mouse AR cDNA encode 252 and 248 amino acid residue type I membrane proteins, respectively. The two proteins share approximately 69% sequence identity. Mouse AR also shares 81% amino acid sequence homology with rat AR. Several secreted isoforms of AR that vary in length and/or glycosylation level can be found in cell conditioned media. The 98 amino acid residue recombinant AR has better receptor binding and biological activity than the C-terminal truncated forms of the protein.
- References:
- Thompson, S.A. et al. (1996) J. Biol. Chem. 271:17927.
- Sonoda, H. et al. (1992) Biochem. Biophys. Res. Commun. 185:103.
- Normanno, N. et al. (2001) Frontiers in Bioscience 6:685.
- Entrez Gene IDs:374 (Human); 11839 (Mouse)
- Alternate Names:Amphiregulin; AR; AREG; AREGB; Colorectum cell-derived growth factor; CRDGF; MGC13647; schwannoma-derived growth factor; SDGF