Recombinant Mouse CHST7 Protein, CF 20 UG

所属行业:生物试剂     有0人感兴趣

Recombinant Mouse CHST7 Protein, CF 20 UG信息二维码

微信扫码

 
基础投资电议
推荐指数
  • 区域全国
  • 留言暂无
  • 浏览0
  • 更新2024-07-25 20:42
  • 到期长期有效






更多>我还有产品
3-Amino-5-methoxycarbonylphenylboronic acid, pinacol ester  1 g 2,3-Dichloro-6-(trifluoromethyl)benzyl bromide  1 g 1-(4-Fluorophenyl)-5-methoxycarbonyl-2(1H)-pyridinone  1 g N,N-Diethyl-cyclohexane-1,4-diamine  1 g N-Methyl-DL-leucine hydrochloride  5 g N-(2-Chloroethyl) 3-boronobenzamide  5 g

产品介绍

    基本参数

    详细说明

    • Purity
      >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
    • Endotoxin Level
      <1.0 EU per 1 μg of the protein by the LAL method.
    • Activity
      Measured by its ability to transfer sulfate from PAPS to N-acetyl-D-glucosamine. The specific activity is >25 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com
    • Source
      Chinese Hamster Ovary cell line, CHO-derived Ser34-Val484, with an N-terminal 6-His tag Accession # Q9EP78
    • Accession #
    • N-terminal Sequence
      Analysis
      His
    • Predicted Molecular Mass
      52 kDa
    • SDS-PAGE
      55-60 kDa, reducing conditions
    5108-ST
     
    Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
    Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 6 months from date of receipt, -20 to -70 °C as supplied.
    • 3 months, -20 to -70 °C under sterile conditions after opening.
    Assay Procedure
    Materials
    • Assay Buffer (provided in kit): 50 mM Tris, 15 mM MgCl2, pH 7.5
    • Recombinant Mouse Carbohydrate Sulfotransferase 7/CHST7 (rmCHST7) (Catalog # 5108‑ST)
    • 3'-Phosphoadenosine-5'-phosphosulfate/PAPS (Catalog # )
    • N-acetyl-alpha -D-glucosamine (GlcNAc)  (Calbiochem, Catalog # 1079), 1 M stock in deionized water
    • Universal Sulfotransferase Activity Kit (Catalog # )
    • 96-well Clear Plate (Costar, Catalog # 92592)
    • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
    1. Dilute 1 mM Phosphate Standard provided by the Universal Sulfotransferase Kit by adding 40 µL of the 1 mM Phosphate Standard to 360 µL of Assay Buffer for a 100 µM stock.
    2. Prepare standard curve by performing six one‑half serial dilutions of the 100 µM Phosphate stock in Assay Buffer. The standard curve has a range of 0.078 to 5 nmol per well.
    3. Prepare reaction mixture containing 0.4 mM PAPS, 30 mM GlcNAc, and 20 μg/mL Coupling Phosphatase 3 in Assay Buffer.
    4. Dilute rmCHST7 to 10 µg/mL in Assay Buffer.
    5. Load 50 µL of each dilution of the standard curve into a plate. Include a curve blank containing 50 μL of Assay Buffer.
    6. Load 25 µL of the 10 µg/mL rmCHST7 into the plate. Include a Control containing 25 µL of Assay Buffer.
    7. Add 25 µL of reaction mixture to the wells, excluding the standard curve.
    8. Cover the plate with a plate sealer and incubate at 37 °C for 20 minutes.
    9. Add 30 µL of the Malachite Green Reagent A to all wells. Mix briefly.
    10. Add 100 µL of deionized water to all wells. Mix briefly.
    11. Add 30 µL of the Malachite Green Reagent B to all wells. Mix and incubate for 20 minutes at room temperature.
    12. Read plate at 620 nm (absorbance) in endpoint mode.
    13. Calculate specific activity:

         Specific Activity (pmol/min/µg) =

    Phosphate released* (nmol) x (1000 pmol/nmol)
    Incubation time (min) x amount of enzyme (µg)

         *Derived from the phosphate standard curve using linear or 4-parameter fitting and adjusted for Control.

    Per Reaction:
    • rmCHST7: 0.25 μg
    • Coupling phosphatase 3: 0.5 μg
    • PAPS: 0.2 mM
    • GlcNAc: 15 mM
    Background: Carbohydrate Sulfotransferase 7/CHST7

    The CHST family is comprised of 14 genes in both human and mouse. All members of this family are Golgi‑localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6‑O or 4‑O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST7, also designated as chondroitin 6‑O‑sulfotransferase (C6ST‑2), N‑acetylglucosamine-6‑O‑sulfotransferase (GlcNAc6ST-4) and Gal/GalNAc/GlcNAc 6‑O‑sulfotransferase (GST‑5), was previously shown to act on chondroitin sulfate, mannose‑linked GlcNAc and mucin associated GlcNAc residues (3‑5). The amino acid sequence of mouse CHST7 is 87% identical to that of the human counterpart. The enzymatic activity of the recombinant mouse CHST7 was measured using a phosphatase-coupled assay (6).

    • References:
      1. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
      2. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
      3. Kitagawa, H. et al. (2000) J. Biol. Chem. 275:21075.
      4. Uchimura, K. et al. (2000) Biochem. Biophys. Res. Commun. 274:291.
      5. Bhakta, S. et al. (2000) J. Biol. Chem. 275:40226.
      6. Prather, B. et al. (2012) Anal. Biochem. 423:86.
    • Entrez Gene IDs:
      56548 (Human); 60322 (Mouse); 302302 (Rat)
    • Alternate Names:
      C6ST2; carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7; Carbohydrate Sulfotransferase 7; CHST7; GlcNAc6ST-4; GST-5
    举报收藏 0评论 0
 
热门项目排行榜快速发布信息
14-(2-碘代乙基)辛基苯 9
2高温波导测试
3格列美脲 98%+ RG  5g
44-溴-3,5-二氯三氟甲苯
5二次谐波
6远红外云试剂
生物试剂排行榜快速发布信息
1Recombinant Human LILR
2Recombinant Human CD47
3Recombinant Human FGF
4203mm具砂板闪式层析柱,
5pUCm-T 载体 5UG
65ml Protein A40 Plus亲
最新加入项目快速发布信息
1质粒大量抽提试剂盒(通
2实验试剂
3产品标题产品标题产品标
4实验试剂
53-Amino-5-methoxycarbo
6N,N-Diethyl-cyclohexan

猜你喜欢产品快速发布信息

相关栏目

还没找到您需要的生物试剂产品?立即发布您的求购意向,让生物试剂公司主动与您联系!

立即发布项目意向

免责声明

本网页所展示的有关【Recombinant Mouse CHST7 Protein, CF 20 UG_生物试剂_云试剂自营四店】的信息/图片/参数等由云试剂的会员【云试剂自营四店】提供,由云试剂会员【云试剂自营四店】自行对信息/图片/参数等的真实性、准确性和合法性负责,本平台(本网站)仅提供展示服务,请谨慎交易,因交易而产生的法律关系及法律纠纷由您自行协商解决,本平台(本网站)对此不承担任何责任。您在本网页可以浏览【Recombinant Mouse CHST7 Protein, CF 20 UG_生物试剂_云试剂自营四店】有关的信息/图片/价格等及提供【Recombinant Mouse CHST7 Protein, CF 20 UG_生物试剂_云试剂自营四店】的商家公司简介、联系方式等信息。

联系方式

在您的合法权益受到侵害时,欢迎您向www@yunshiji.cn邮箱发送邮件,或者进入《网站意见反馈》了解投诉处理流程,我们将竭诚为您服务,感谢您对云试剂的关注与支持!

按排行字母分类找加盟项目:

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z