详细说明
- Purity>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<1.0 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to cleave Recombinant Human Aggrecan G1-IGD-G2 Domains (Catalog # ). rmADAMTS1 achieves >50% substrate cleavage, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com
- SourceChinese Hamster Ovary cell line, CHO-derived Phe254-Cys725, with a C-terminal 10-His tag
- Accession #
- N-terminal Sequence
AnalysisPhe254 - Structure / FormMonomer. Recombinant Mouse ADAMTS1 is prone to proteolytic cleavage at C-terminus. The predominant form of the purified protein lacks the His tag.
- Predicted Molecular Mass53 kDa
- SDS-PAGE65-70 kDa, reducing conditions
5867-AD | | |
Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate, CaCl 2 and NaCl. | ||
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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- Assay Buffer: 25 mM HEPES, 0.01% (w/v) Brij-35, pH 7.0
- Recombinant Human Aggrecan Aggrecan G1-IGD-G2 Domains (rhAggrecan) (Catalog # )
- Recombinant Mouse ADAMTS1 Recombinant Mouse ADAMTS1 (rmADAMTS1) (Catalog # 5867-AD)
- SDS-PAGE and/or Western blot
- Dilute rhAggrecan to 200 µg/mL in Assay Buffer.
- Dilute rmADAMTS1 to 20 µg/mL in Assay Buffer.
- Combine 25 µL of 200 µg/mL rhAggrecan with 25 µL of 20 µg/mL rmADAMTS1 in a reaction tube.
- Incubate the reaction mixture for 24 hours at 37 °C.
- For Controls: Combine 25 µL of rhAggrecan solution and 25 µL of Assay Buffer into two tubes. Incubate one tube at 37 °C and the other at -20 °C for 24 hours.
- Stop the reaction by adding reduced SDS-PAGE sample buffer.
- Analyze the cleavage by SDS PAGE followed by protein staining and/or Western blot.
- rmADAMTS1: 10 µg/mL (0.5 µg)
- rhAggrecan: 100 µg/mL (5 µg)
- References:
- Vazquez, F. et al. (1999) J. Biol. Chem. 274:23349.
- Kuno, K. et al. (1997) J. Biol. Chem. 272:556.
- Porter, S. et al. (2005) Biochem. J. 386:15.
- Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
- Kuno, K. et al. (1999) J. Biol. Chem. 274:18821.
- Kuno, K. et al. (2000) FEBS Lett. 478:241.
- Russel, D. L. et al. (2003) J. Biol. Chem. 278:42330.
- Lee, N. et al. (2005) J. Biol. Chem. 280:34796.
- Shindo, T. et al. (2000) J. Clin. Invest. 105:1345.
- Wight, T.N. (2005) Arterioscler Thromb. Vasc. Biol. 25:12.
- Luque, A. et al. (2003) J. Biol. Chem. 278:23656.
- Krampert, M. et al. (2005) J. Biol. Chem. 280:23844.
- Long Name:A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 1
- Entrez Gene IDs:9510 (Human)
- Alternate Names:a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1; ADAM metallopeptidase with thrombospondin type 1 motif, 1; ADAM-TS 1; ADAMTS1; ADAM-TS1; ADAMTS-1; C3-C5; METH1; METH-1