Recombinant Mouse ADAMTS1 Protein, CF 20 UG

所属行业:生物试剂     有0人感兴趣

Recombinant Mouse ADAMTS1 Protein, CF 20 UG信息二维码

微信扫码

 
基础投资电议
推荐指数
  • 区域全国
  • 留言暂无
  • 浏览0
  • 更新2024-07-25 20:42
  • 到期长期有效






更多>我还有产品
3-Amino-5-methoxycarbonylphenylboronic acid, pinacol ester  1 g 2,3-Dichloro-6-(trifluoromethyl)benzyl bromide  1 g 1-(4-Fluorophenyl)-5-methoxycarbonyl-2(1H)-pyridinone  1 g N,N-Diethyl-cyclohexane-1,4-diamine  1 g N-Methyl-DL-leucine hydrochloride  5 g N-(2-Chloroethyl) 3-boronobenzamide  5 g

产品介绍

    基本参数

    详细说明

    • Purity
      >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
    • Endotoxin Level
      <1.0 EU per 1 μg of the protein by the LAL method.
    • Activity
      Measured by its ability to cleave Recombinant Human Aggrecan G1-IGD-G2 Domains (Catalog # ). rmADAMTS1 achieves >50% substrate cleavage, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com
    • Source
      Chinese Hamster Ovary cell line, CHO-derived Phe254-Cys725, with a C-terminal 10-His tag
    • Accession #
    • N-terminal Sequence
      Analysis
      Phe254
    • Structure / Form
      Monomer. Recombinant Mouse ADAMTS1 is prone to proteolytic cleavage at C-terminus. The predominant form of the purified protein lacks the His tag.
    • Predicted Molecular Mass
      53 kDa
    • SDS-PAGE
      65-70 kDa, reducing conditions
    5867-AD
     
    Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate, CaCl 2 and NaCl.
    Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 6 months from date of receipt, -20 to -70 °C as supplied.
    • 3 months, -20 to -70 °C under sterile conditions after opening.
    Assay Procedure
    Materials
    • Assay Buffer: 25 mM HEPES, 0.01% (w/v) Brij-35, pH 7.0
    • Recombinant Human Aggrecan Aggrecan G1-IGD-G2 Domains (rhAggrecan) (Catalog # )
    • Recombinant Mouse ADAMTS1 Recombinant Mouse ADAMTS1 (rmADAMTS1) (Catalog # 5867-AD)
    • SDS-PAGE and/or Western blot
    1. Dilute rhAggrecan to 200 µg/mL in Assay Buffer.
    2. Dilute rmADAMTS1 to 20 µg/mL in Assay Buffer.
    3. Combine 25 µL of 200 µg/mL rhAggrecan with 25 µL of 20 µg/mL rmADAMTS1 in a reaction tube.
    4. Incubate the reaction mixture for 24 hours at 37 °C.
    5. For Controls: Combine 25 µL of rhAggrecan solution and 25 µL of Assay Buffer into two tubes. Incubate one tube at 37 °C and the other at -20 °C for 24 hours.
    6. Stop the reaction by adding reduced SDS-PAGE sample buffer.
    7. Analyze the cleavage by SDS PAGE followed by protein staining and/or Western blot.
    Per Reaction:
    • rmADAMTS1: 10 µg/mL (0.5 µg)
    • rhAggrecan: 100 µg/mL (5 µg)
    Background: ADAMTS1
    ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1), also known as METH1, is the founding member of the family of secreted zinc proteases with a multidomain structure (1-3). The protein precursor consists of a signal peptide and the following domains: pro, catalytic, disintegrinlike, TS type 1 motif, cysteine rich, spacer and a variable number of thrombospondin type 1 motifs. Based on their substrate specificity, ADAMTS1 and associated family members may be key enzymes in the degradation of cartilage leading to inflammation and arthritis (4). It is an active protease cleaving alpha 2macroglobulin (5), aggrecan (6), and versican (7). Compared to ADAMTS4 (aggrecanase 1) and ADAMTS5 (aggrecanase 2), the aggrecanase activity of ADAMTS1 is lower. However, its activity can be enhanced by the binding of a cofactor such as fibulin1 (8). ADAMTS1 is essential for normal growth and the structure and function of the kidneys, adrenal glands and female reproductive organs (9). It also plays an important role in atherosclerosis (10). It has been shown to inhibit endothelial cell proliferation by direct binding and sequestration of VEGF165 and to inhibit fibroblast migration at high concentrations by binding to FGF 2 (11, 12). The purified rmADAMTS1 starts at the N terminus of the catalytic domain and ends in the Cys-rich domain.
    • References:
      1. Vazquez, F. et al. (1999) J. Biol. Chem. 274:23349.
      2. Kuno, K. et al. (1997) J. Biol. Chem. 272:556.
      3. Porter, S. et al. (2005) Biochem. J. 386:15.
      4. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
      5. Kuno, K. et al. (1999) J. Biol. Chem. 274:18821.
      6. Kuno, K. et al. (2000) FEBS Lett. 478:241.
      7. Russel, D. L. et al. (2003) J. Biol. Chem. 278:42330.
      8. Lee, N. et al. (2005) J. Biol. Chem. 280:34796.
      9. Shindo, T. et al. (2000) J. Clin. Invest. 105:1345.
      10. Wight, T.N. (2005) Arterioscler Thromb. Vasc. Biol. 25:12.
      11. Luque, A. et al. (2003) J. Biol. Chem. 278:23656.
      12. Krampert, M. et al. (2005) J. Biol. Chem. 280:23844.
    • Long Name:
      A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 1
    • Entrez Gene IDs:
      9510 (Human)
    • Alternate Names:
      a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 1; ADAM metallopeptidase with thrombospondin type 1 motif, 1; ADAM-TS 1; ADAMTS1; ADAM-TS1; ADAMTS-1; C3-C5; METH1; METH-1
    举报收藏 0评论 0
 
热门项目排行榜快速发布信息
14-(2-碘代乙基)辛基苯 9
2高温波导测试
3格列美脲 98%+ RG  5g
44-溴-3,5-二氯三氟甲苯
5二次谐波
6远红外云试剂
生物试剂排行榜快速发布信息
1Recombinant Human LILR
2Recombinant Human CD47
3Recombinant Human FGF
4203mm具砂板闪式层析柱,
5pUCm-T 载体 5UG
65ml Protein A40 Plus亲
最新加入项目快速发布信息
1质粒大量抽提试剂盒(通
2实验试剂
3产品标题产品标题产品标
4实验试剂
53-Amino-5-methoxycarbo
6N,N-Diethyl-cyclohexan

猜你喜欢产品快速发布信息

相关栏目

还没找到您需要的生物试剂产品?立即发布您的求购意向,让生物试剂公司主动与您联系!

立即发布项目意向

免责声明

本网页所展示的有关【Recombinant Mouse ADAMTS1 Protein, CF 20 UG_生物试剂_云试剂自营四店】的信息/图片/参数等由云试剂的会员【云试剂自营四店】提供,由云试剂会员【云试剂自营四店】自行对信息/图片/参数等的真实性、准确性和合法性负责,本平台(本网站)仅提供展示服务,请谨慎交易,因交易而产生的法律关系及法律纠纷由您自行协商解决,本平台(本网站)对此不承担任何责任。您在本网页可以浏览【Recombinant Mouse ADAMTS1 Protein, CF 20 UG_生物试剂_云试剂自营四店】有关的信息/图片/价格等及提供【Recombinant Mouse ADAMTS1 Protein, CF 20 UG_生物试剂_云试剂自营四店】的商家公司简介、联系方式等信息。

联系方式

在您的合法权益受到侵害时,欢迎您向www@yunshiji.cn邮箱发送邮件,或者进入《网站意见反馈》了解投诉处理流程,我们将竭诚为您服务,感谢您对云试剂的关注与支持!

按排行字母分类找加盟项目:

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z