• Purity

  >90%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit proliferation of PC‑3 human prostate cancer cells. The ED    ₅₀ for this effect is 5-30 ng/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Mouse Ephrin-A2 (Glu23-Asn184)  Accession # P52801	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Glu23

• Structure / Form

  Disulfied-linked homodimer

• Predicted Molecular Mass

  46 kDa

• SDS-PAGE

  52-61 kDa, reducing conditions

Background: Ephrin-A2

Ephrin-A2, also known as ELF-1, HEK7-L, LERK-6, and EPLG6, is an approximately 20 kDa member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-A2 preferentially interacts with receptors in the EphA family (1, 2). Mouse Ephrin-A2 is synthesized with a 20 amino acid (aa) signal peptide, a 164 aa mature chain, and a 25 aa C-terminal propeptide which is removed prior to GPI linkage of Ephrin-A2 to the membrane (3, 4). It shares 93% and 100% aa sequence identity with human and rat Ephrin-A2, respectively. Ephrin-A2 is expressed in discrete regions of the developing nervous system and limb buds (4-7). Its distribution complements the pattern of Eph receptor expression, and this plays an important role in tissue morphogenesis (7-9). Ephrin-A2 exerts an axon repulsive signal which is important for the accurate pathfinding of retinal ganglion cell axons to the tectum and hippocampal axons to the lateral septum (8, 10). Its up-regulation in astrocytes at sites of optic nerve damage may prevent re-innervation by retinal ganglion cells (11). Ephrin-A2 is also expressed on neural progenitor cells in the subventricular zone (SVZ). It interacts with EphA7, triggering reverse signaling through Ephrin-A2 and inhibition of progenitor cell proliferation (9). In the developing limbs, Ephrin-A2 regulates cartilage morphogenesis and the projection of motoneuron axons (6, 7, 12).

• References:

1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.

2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.

3. Shao, H. et al. (1995) J. Biol. Chem. 270:3467.

4. Cheng, H.-J., and J.G. Flanagan (1994) Cell 79:157.

5. Kenmuir, C.L. et al. (2012) Anat. Rec. (Hoboken) 295:105.

6. Ohta, K. et al. (1997) Mech. Dev. 64:127.

7. Wada, N. et al. (2003) Dev. Biol. 264:550.

8. Gao, P.-P. et al. (1996) Proc. Natl. Acad. Sci. USA 93:11161.

9. Holmberg, J. et al. (2005) Genes Dev. 19:462.

10. Nakamoto, M. et al. (1996) Cell 86:755.

11. Symonds, A.C.E. et al. (2007) Eur. J. Neurosci. 25:744.

12. Eberhart, J. et al. (2000) Dev. Neurosci. 22:237.

• Entrez Gene IDs:

  1943 (Human); 13637 (Mouse)

• Alternate Names:

  Cek7-L; EFNA2; ELF-1; EPH-related receptor tyrosine kinase ligand 6; EphrinA2; Ephrin-A2; EPLG6HEK7 ligand; HEK7-L; HEK7-ligand; LERK6; LERK6LERK-6; ligand of eph-related kinase 6