详细说明
- Purity>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its binding ability in a functional ELISA. Immobilized recombinant rat UNC5H2 Fc Chimera at 5 µg/mL (100 µL/well) can bind Recombinant Mouse Netrin-1 with a linear range of 3‑200 ng/mL.
- SourceMouse myeloma cell line, NS0-derived
Mouse Netrin-1
(Val22-Ala603)
Accession # AAC52971IEGR GGGSGGGSGGGS 10-His tag N-terminus C-terminus - Accession #
- N-terminal Sequence
AnalysisVal22 - Predicted Molecular Mass68.2 kDa
- SDS-PAGE83 kDa, reducing conditions
1109-N1 | | 1109-N1/CF |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with Trehalose. | |
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
SDS-PAGE | 1 μg/lane of Recombinant Mouse Netrin-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 83 kDa. |
- References:
- Puschel, A. (1999) Mech. Dev. 83:65.
- Hedgecock, E. and C. Norris (1997) Trends Genet. 13:251.
- Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287.
- Madison, R. et al. (2000) Exp. Neurology 161:563.
- Srinivasan, K. et al. (2003) Dev. Cell 4:371.
- Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
- Corset, V. et al. (2000) Nature 407:747.
- Entrez Gene IDs:9423 (Human); 18208 (Mouse); 396389 (Chicken)
- Alternate Names:netrin 1; Netrin1; Netrin-1; NTN1; NTN1Lnetrin 1, mouse, homolog of