详细说明
- Purity>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<1.0 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED 50 for this effect is 50-150 ng/mL.
- SourceE. coli-derived Leu53-Ala103
- Accession #
- N-terminal Sequence
AnalysisLeu53 - Predicted Molecular Mass5.9 kDa
1127-EP | | 1127-EP/CF |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | |
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Epigen is an eipthelial mitogen that belongs to the EGF superfamily (1). Mouse Epigen cDNA encodes a 153 amino acid (aa) residue type I transmembrane precursor protein with a 18 aa putative signal peptide, a 92 aa extracellular domain, a 20 aa transmembrane domain and a 22 aa cytoplasmic domain. Expression of mouse Epigen has been detected in multiple tissues including testis, liver and heart. Over the entire protein, mouse Epigen shares from approximately 23% to 33% sequence homology with other EGF family members. The functional internal peptide containing the EGF motif however is more than 40% homologous with that of TGF-alpha and epiregulin. A human cDNA clone encoding a mouse epigen-like protein has also been identified (2). Similarly to other EGF family proteins, a soluble form of mouse Epigen can most likely be generated from the membrane-bound form by proteolytic cleavage. Although recombinant mouse Epigen has been shown to be mitogenic for fibroblasts (3) and epithelial cells (1), compared to EGF and TGF-alpha, it is much less potent. The EGF receptor ErbB1 can be activated upon mouse Epigen activation. Nevertheless, involvements of additional ErbB receptors in mouse Epigen signaling have not been ruled out.
- References:
- Strachan, L. et al. (2001) J. Biol. Chem. 276:18265.
- Accession XM_171078.
- R&D Systems, unpublished results.
- Entrez Gene IDs:255324 (Human); 71920 (Mouse)
- Alternate Names:EPGN; Epigen