详细说明
- Purity>80%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<0.1 EU per 1 μg of the protein by the LAL method.
- Activity
Measured by its ability to bind rrUNC5H2/Fc Chimera in a functional ELISA.
Immobilized rrUNC5H2/Fc Chimera at 5 µg/mL (100 µL/well) can bind rmNetrin-4 with a linear range of 6-400 ng/mL.
- SourceMouse myeloma cell line, NS0-derived Leu19-Val628 & Ala26-Val628, both with a C-terminal 10-His tag
- Accession #
- N-terminal Sequence
AnalysisLeu19 & Ala26 - Predicted Molecular Mass69 kDa
- SDS-PAGE85 kDa, reducing conditions
1132-N4 | | 1132-N4/CF |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | |
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Netrins/UNC-6 (netr: Sanskrit for “one who guides”) are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins binds to the DCC and UNC5 family of receptors to attract or repel axons. Mouse Netrin-4, also known as beta -Netrin, is synthesized as a 629 amino acid (aa) precursor that contains a 19 aa signal sequence, a 428 aalaminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a 182 aa C domain rich in basic aa residues that serves as a heparin binding site. Netrin-4 has been reported to exist as both a monomer and a dimer. The dimeric form was reported to be the less active of the two. Mouse Netrin-4 shares 31%, 29% and 25% aa sequence identity with mouse Netrin-1, -3, and -G1a, respectively. It also shares 89% aa sequence identity with human Netrin-4. Netrin-4 has widespread expression, occurring in nervous tissues such as embryonic floor plate and postnatal neurons such as cerebellar granule cells and hippocampal pyramidal cells. It also is found in non-neural tissues such as adult Bowman’s capsule and medullary tubular epithelium in kidney, and embryonic pancreatic and intestinal epithelium, plus cells of the ureteric bud. Netrin-4 expression is often associated with basement membrane. Netrin-4 has been shown to initiate axon outgrowth from olfactory bulb explants (1 - 7).
- References:
- McFarlane, S. (2000) Biochem. Cell. Biol. 78:563.
- Kennedy, T.E. (2000) Biochem. Cell Biol. 78:569.
- Yu, T.W. and C.I. Bargmann (2001) Nature Neurosci. 4(Suppl):1169.
- Stein, E. et al. (2001) Science 291:1976.
- Koch, M. et al. (2000) J. Cell Biol. 151:221.
- Yin, Y. et al. (2000) Mech. Dev. 96:115.
- Livesey, F.J. (1999) Cell. Mol. Life Sci. 56:62.
- Entrez Gene IDs:59277 (Human); 57764 (Mouse)
- Alternate Names:beta-Netrin; hepar-derived netrin-like protein; netrin 4; Netrin4; Netrin-4; NTN4; PRO3091