• Purity

  >97%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Bioassay data are not available.

• Source

  Spodoptera frugiperda,     Sf 21 (baculovirus)-derived His29-Gly513, with a C-terminal 10-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  His29

• Predicted Molecular Mass

  56 kDa

• SDS-PAGE

  70-75 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Netrin-G1a

Netrins/UNC-6 (netr: Sanskrit for “one who guides”) are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins bind to the DCC and UNC5 family of receptors to attract or repel axons. Mouse Netrin-G1a is synthesized as a 539 amino acid (aa) precursor with an 18 aa signal sequence, a 399 aa laminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a 122 aa C domain with a hydrophobic signal for glycosyl phosphatidylinositol (GPI) lipid linkage. Unlike classical Netrins which have a C domain rich in basic aa residues that serves as a heparin binding site, Netrin-G1a is predominantly anchored on the cell surface via GPI linkages. By alternative splicing, at least six isoforms for Netrin-G1 have been identified. All G1a variants (G1b through G1f) are shorter than G1a and lack one or more of the EGF-like domains. Mouse Netrin-G1a shares 27%, 26% and 31% aa sequence identity with mouse Netrins-1, 3 and 4, respectively. It also shares 60% aa identity with another GPI-anchored mouse Netrin-G2a. Although a human homolog of mouse Netrin-G1a has not been reported, a human G1f that shares 97% aa sequence identity with mouse G1f has been cloned. Netrin-G1 has widespread expression, occurring in olfactory mitral cells, cells of the inferior colliculus (hearing), dorsal thalamus (behavior), and cells of the deep cerebellar nuclei and inferior olive (motion). The G-type Netrins are found only in vertebrates and are suggested to play a role in forming circuits associated with motor activity. Conditioned media containing myc-tagged Netrin-G1a proteins did not bind to cells transiently transfected with the Netrin receptors DCC, UNC5H1, UNC5H2, or UNC5H3 (1 - 4).

• References:

1. McFarlane, S. (2000) Biochem. Cell. Biol. 78:563.

2. Yu, T.W. and C.I. Bargmann (2001) Nature Neurosci. 4(Suppl):1169.

3. Nakashiba, T. et al. (2002) Mech. Dev. 111:47.

4. Nakashiba, T. et al. (2000) J. Neurosci. 20:6540.

• Entrez Gene IDs:

  22854 (Human); 80883 (Mouse)

• Alternate Names:

  NetrinG1a; Netrin-G1a; NTG1; NTNG1