详细说明
- Purity>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to compete with Frizzled-1 for binding to biotinylated Wnt-3a. The IC 50 value is 0.7-3.5 nM, under conditions in which Recombinant Mouse (rm) Frizzled‑1 Fc Chimera (Catalog # ) is present at 2.1 nM, and biotinylated rmWnt-3a concentration is 2.7 nM.
- SourceMouse myeloma cell line, NS0-derived Leu25-Cys295, with a C-terminal 6-His tag
- Accession #
- N-terminal Sequence
AnalysisLeu25 - Predicted Molecular Mass31.6 kDa
- SDS-PAGE35-40 kDa, reducing conditions
1169-FR/CF | | 1169-FR |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | |
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
- References:
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- Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
- Hu, E. et al. (1998) Biochem. Biophys. Res. Commun. 247:287.
- Leimeister, C. et al. (1998) Mech. Dev. 75:29.
- Galli, L.M. et al. (2006) Dev. Dyn. 235:681.
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- von Marschall, Z. and L.W. Fisher (2010) Biochem. Biophys. Res. Commun. 400:299.
- Oshima, T. et al. (2005) Blood 106:3160.
- Lee, J.-L. et al. (2004) J. Biol. Chem. 279:14602.
- Sugiyama, Y. et al. (2010) Dev. Biol. 338:193.
- Descamps, S. et al. (2008) Cell Tissue Res. 332:299.
- Roth, W. et al. (2000) Oncogene 19:4210.
- He, W. et al. (2010) Proc. Natl. Acad. Sci. 107:21110.
- Kobayashi, K. et al. (2009) Nat. Cell Biol. 11:46.
- Long Name:Secreted Frizzled Related Protein 2
- Entrez Gene IDs:6423 (Human); 20319 (Mouse)
- Alternate Names:FRP2; FRP-2secreted apoptosis related protein 1; SARP-1; SARP1sFRP-2; SDF-5; Secreted apoptosis-related protein 1; secreted frizzled-related protein 2; sFRP2; sFRP-2