详细说明
- Purity>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.01 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED 50 for this effect is 0.25-1.5 ng/mL.
- SourceE. coli-derived Val17-Ala169, with and without an N-terminal Met
- Accession #
- N-terminal Sequence
AnalysisVal17 and Met - Structure / FormDisulfide-linked homodimer
- Predicted Molecular Mass17.6 kDa
1399-IL | | 1399-IL/CF |
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. | |
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. | Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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- References:
- Saadoun, D. et al. (2011) Curr. Pharm. Des. 17:3781.
- Iwakura, Y. et al. (2011) Immunity 34:149.
- Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
- Fort, M.M. et al. (2001) Immunity 15:985.
- Zhao, A. et al. (2010) J. Immunol. 185:6921.
- Suzukawa, M. et al. (2012) J. Immunol. 189:3641.
- Corrigan, C.J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:1579.
- Petersen, B.C. et al. (2012) Nat. Med. 18:751.
- Wang, Y.-H. et al. (2007) J. Exp. Med. 204:1837.
- Rickel, E.A. et al. (2008) J. Immunol. 181:4299.
- Hurst, S.D. et al. (2002) J. Immunol. 169:443.
- Kleinschek, M.A. et al. (2007) J. Exp. Med. 204:161.
- Cao, Q. et al. (2011) J. Am. Soc. Nephrol. 22:1229.
- Stock, P. et al. (2009) J. Immunol. 182:5116.
- Caruso, R. et al. (2009) Gastroenterology 136:2270.
- Goswami, S. et al. (2009) Nat. Immunol. 10:496.
- Sonobe, Y. et al. (2009) J. Biol. Chem. 284:31834.
- Long Name:Interleukin 17E
- Entrez Gene IDs:64806 (Human); 140806 (Mouse); 501996 (Rat)
- Alternate Names:IL17E; IL-17E; IL17Einterleukin-17E; IL25; IL-25; interleukin 17E; interleukin 25; Interleukin-17E; interleukin-25