详细说明
- Purity>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<1.0 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED 50 for this effect is 2-6 µg/mL.
- SourceMouse myeloma cell line, NS0-derived Leu21-Leu230, with a C-terminal 10-His tag
- Accession #
- N-terminal Sequence
AnalysisLeu21 - Structure / Formmonomer
- Predicted Molecular Mass26.8 kDa
- SDS-PAGE40 kDa, reducing conditions
1508-S1/CF | | 1508-S1 |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | |
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Soggy-1, also known as DkkL1 (Dickkopf-like 1), is a secreted protein that is related to the Dickkopf family of Wnt antagonists. Dkk-1, -2, -3, and -4 each have two cysteine-rich domains separated by a linker. Soggy-1 does not contain cysteine-rich domains but bears some sequence homology with the N-terminal regions of Dkk proteins (1, 2). The mouse Soggy-1 cDNA encodes a 230 amino acid (aa) precursor that includes a 20 aa signal sequence (2). Mouse Soggy-1 shares 65% and 90% aa sequence identity with human and rat Soggy-1, respectively, and approximately 15% aa sequence identity with mouse Dkk-1, -2, -3, and -4. Mouse Soggy-1 is expressed at various sites in the embryo but in the adult is primarily found in the testes (3 - 5). Soggy-1 transcription is regulated by the spermatocyte specific factor, RFX2 (6). The regulatory elements for Soggy-1 lie very close to those of TEAD-2, a transcription factor that is expressed very early in development (4). Soggy-1 and TEAD-2 are co-expressed in preimplantation embryos and embryonic stem cells, but differentiated cells express only one or the other (4, 5). During development, Soggy-1 is first detectable at the onset of sexual differentiation (4). Soggy-1 is localized to the acrosome in developing spermatocytes and mature spermatozoa (3, 4). Soggy-1, as expressed in developing mouse spermatocytes, is a 34 kDa N-glycosylated protein. This glycosylation is not present on Soggy-1 in mature spermatozoa, although the apparent molecular weight suggests the presence of some post-translational modification (3). Two shorter forms of Soggy-1 have been described that result from the use of internal methionine residues for initiation (4).
- References:
- Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
- Krupnik, V.E. et al. (1999) Gene 238:301.
- Kohn, M.J. et al. (2005) Mol. Reprod. Dev. 71:516.
- Kaneko, K.J. and M.L. DePamphilis (2000) Nucleic Acids Res. 28:3982.
- Kaneko, K.J. et al. (2004) Mol. Cell. Biol. 24:1968.
- Horvath, G.C. et al. (2004) Biol. Reprod. 71:1551.
- Long Name:Dickkopf-like Protein 1
- Entrez Gene IDs:27120 (Human); 50722 (Mouse)
- Alternate Names:Cancer/testis antigen 34; CT34dickkopf-like 1 (soggy); dickkopf-like 1; DkkL1; Protein soggy-1; SGY; SGY1; SGY-1dickkopf-like protein 1; SGY1soggy; Soggy1; Soggy-1