详细说明
- Purity>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to inhibit rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Thies, R.S. et al. (2001) Growth Factors 18:251. The ED 50 for this effect is 0.05-0.25 µg/mL in the presence of 5 ng/mL of Recombinant Mouse GDF‑8/Myostatin. Approximately 1 µg/mL will completely inhibit GDF-8 activity in these cells.
- SourceMouse myeloma cell line, NS0-derived Asn25-Ser265, with a C-terminal 10-His tag
- Accession #
- N-terminal Sequence
AnalysisAsn25 - Predicted Molecular Mass28.7 kDa
- SDS-PAGE39 kDa, reducing conditions
1539-PG/CF | | 1539-PG |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | |
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
- References:
- McPherron, A.C. (2010) Immunol. Endocr. Metab. Agents Med. Chem. 10:217.
- McPherron, A.C. et al. (1997) Nature 387:83.
- Zimmers, T.A. et al. (2002) Science 296:1486.
- Wolfman, N.M. et al. (2003) Proc. Natl. Acad. Sci. 100:15842.
- McFarlane, C. et al. (2005) Dev. Biol. 283:58.
- Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. 98:9306.
- Thies, R.S. et al. (2001) Growth Factors 18:251.
- Amthor, H. et al. (2004) Dev. Biol. 270:19.
- Hill, J.J. et al. (2002) J. Biol. Chem. 277:40735.
- Miura, T. et al. (2006) Biochem. Biophys. Res. Commun. 340:675.
- Hill, J.J. et al. (2003) Molecular Endocrinology 17:1144.
- Anderson, S.B. et al. (2008) J. Biol. Chem. 283:7027.
- Rebbapragada, A. et al. (2003) Mol. Cell. Biol. 23:7230.
- Guo, W. et al. (2008) J. Biol. Chem. 283:9136.
- Matsakas, A. et al. (2009) Neuromuscul. Disord. 19:489.
- Guo, T. et al. (2009) PloS ONE 4:e4937.
- Long Name:Growth Differentiation Factor 8
- Entrez Gene IDs:2660 (Human); 17700 (Mouse)
- Alternate Names:GDF8; GDF-8; GDF8growth differentiation factor 8; growth/differentiation factor 8; MSTN; Myostatin