详细说明
- Purity>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<1.0 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its binding ability in a functional ELISA. Immobilized rhC1q at 5 µg/mL (100 µL/well) can bind rmPentraxin 3 with a linear range of 0.25-15 µg/mL.
- SourceMouse myeloma cell line, NS0-derived Glu18-Ser381, with a C-terminal 6-His tag
- Accession #
- N-terminal Sequence
AnalysisGlu18 - Structure / FormMultimer consisting of as many as ten non-covalently and covalently linked subunits
- Predicted Molecular Mass41 kDa (monomer)
- SDS-PAGE46 kDa, reducing conditions
| 2166-TS | | 2166-TS/CF |
| Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CaCl 2 with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CaCl 2. | |
| Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | |
| Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Pentraxin 3, also known as TSG-14, was initially identified as a TNF-alpha or IL-1 beta inducible gene (1 - 3). It belongs to the pentraxin family, which was named originally for the homo-pentameric structure formed by its members (4). The pentraxin family is divided into two subfamilies: the “short” and the “long” pentraxins with approximate molecular weights of 25 kDa and 50 kDa, respectively. TSG-14 is a member of the long pentraxin subfamily, which also includes the Xenopus laevis XL-PXN1, the guinea pig apexin/p50, the rat neuronal pentraxin I (NPI) and NPR, the human neuronal pentraxin II (NPTX2) and the human neuronal activity-related pentraxin (5).
Mature secreted PTX3 contains a pentaxin-like domain at its carboxy-terminus that shares 23 - 28% amino acid (aa) sequence similarity to C-reactive protein (CRP) and serum amyloid P component (SAP), which belong to the short pentraxin subfamily. However, the N-terminal sequence of TSG-14 does not share aa sequence homology with any of the “short” pentaxins (3). Unlike CRP and SAP, which forms pentamers only, TSG-14 forms both pentameric and higher ordered oligomers (5). Similar to CRP and SAP, TSG-14 binds to the complement cascade component C1q (6). However, TSG-14 does not bind to phosphoethanolamine, phosphocholine, or high pyruvate agarose, which are known ligands for CRP and SAP. While CRP and SAP are primarily produced in the liver, TSG-14 expression is strongly upregulated by TNF-alpha, IL-1 beta, and bacterial LPS in peripheral fibroblasts, endothelial cells, and macrophages (7). At the amino acid level, human and mouse TSG-14 share 88% aa sequence homology (8). TSG-14 concentration is elevated in the joint fluid of patients with rheumatoid arthritis (RA), indicating that TSG-14 may be a potential mediator of immune response (9). TSG-14 may also function in the regulation of the uptake and clearance of apoptotic cells by dendritic cells (10). An in vivo study showed that TSG-14 transgenic mice are more resistant to sepsis and endotoxemia compared to wild-type during inflammatory injury (11). Increased expression of TSG-14 may enhance the immune response to protect the host from infection.
- References:
- Lee, T.H. et al. (1990) Mol. Cell. Biol. 10:1982.
- Breviario, F. et al. (1992) JBC. 267:22190.
- Lee, G.W. et al. (1993) J. Immunol. 150:1804.
- Osmand, A.P. et al. (1977) PNAS, USA 74:739.
- Goodman A.R. et al. (1996) Cytokine & Growth Factor Rev. 7:191.
- Bottazzi, B. et al. (1997) JBC. 272:32817.
- Introna, M. et al. (1996) Blood, 87:1862.
- Altmeyer, A. et al. (1995) J. Biol. Chem. 270:25584.
- Luchetti, M.M. et al. (2000) Clin. Exp. Immunol. 119:196.
- Rovere, P. et al. (2000) Blood 96:4300.
- Dias, A.A.M. et al. (2001) J. Leukocyte Biol. 69:928.
- Long Name:Tumor Necrosis Factor Stimulated Gene 14
- Entrez Gene IDs:5806 (Human); 19288 (Mouse)
- Alternate Names:alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14
400-6226-992