详细说明
- Purity>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its binding ability in a functional ELISA. Immobilized recombinant mouse IL-36 alpha at 1 µg/mL (100 µL/well) can bind recombinant mouse IL-1 Rrp2 (Catalog # 2354-RP) with a linear range of 0.15 ‑ 5 µg/mL.
- SourceE. coli-derived Met1-His160
- Accession #
- N-terminal Sequence
AnalysisMet1 - Predicted Molecular Mass21 kDa
| 2297-ML | | 2297-ML/CF |
| Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | |
| Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | |
| Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Mouse interleukin‑36 alpha [IL‑36 alpha ; previously IL‑1F6 and also named FIL‑1 epsilon (epsilon)] is a member of the IL‑1 family of proteins (1 ‑ 3, 7). IL‑1 family members include IL‑1 beta, IL‑1 alpha, IL‑1ra, IL‑18, IL‑36Ra/IL‑1F5, IL‑37/IL‑1F7, IL‑36 beta /IL‑1F8, IL‑36 gamma /IL‑1F9 and IL‑1F10 (4, 7). All family members show a 12 beta ‑strand, beta ‑trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL‑36 alpha /IL‑1F6 is synthesized as a 160 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N‑linked glycosylation site(s) (2, 5). It appears to be actively secreted (1). When found in cell lysates, it presents as an 18 kDa monomer (2). Mouse to human, full length IL‑36 alpha /IL‑1F6 has 54% aa identity. Within the family, IL‑36 alpha /IL‑1F6 is 29% aa identical to IL‑1ra, and 32%, 32%, 34%, 41%, and 28% aa identical to IL‑1 beta, IL‑36Ra/IL‑1F5, IL‑36 beta /IL‑1F8, IL‑36 gamma /IL‑1F9 and IL‑1F10, respectively. Cells reported to express IL‑36 alpha /IL‑1F6 include monocytes, B cells and T cells (1, 4). Notably, IL‑36 alpha /IL‑1F6 is the only novel IL‑1 family member found to be expressed on T cells. The receptor for IL‑36 alpha /IL‑1F6 is reported to be a combination of IL‑1 Rrp2 and IL‑1 RAcP (6). Recombinant IL‑36 alpha /IL‑1F6, along with IL‑36 beta /IL‑1F8 and IL‑36 gamma /IL‑1F9, has been shown to act as an agonist by activating the pathway involving NF‑ kappa B and MAPK in an IL‑1 Rrp2 dependent manner. This suggests that IL‑36 alpha /IL‑1F6 may signal in similar fashion to IL‑1 and IL‑18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.
- References:
- Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
- Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
- Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
- Dunn, E. et al. (2001) Trends Immunol. 22:533.
- Debets, R. et al. (2001) J. Immunol. 167:1440.
- Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
- Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
- Long Name:Interleukin 36 alpha/Interleukin 1 Family 6
- Entrez Gene IDs:27179 (Human); 54448 (Mouse)
- Alternate Names:FIL1 epsilon; FIL1; FIL1(EPSILON); FIL1E; IL-1 epsilon; IL1(EPSILON); IL1E; IL-1E; IL-1F6 (FIL-1-epsilon); IL1F6; IL-1F6; IL36 alpha; IL-36 alpha; IL36A; interleukin 1 family, member 6 (epsilon); interleukin 1, epsilon; Interleukin 36, Alpha; Interleukin-1 epsilon; interleukin-1 family member 6; Interleukin-36 Alpha
400-6226-992