• Purity

  >97%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse IL-36 beta at 1 µg/mL (100 µL/well) can bind recombinant mouse IL-1 Rrp2 (Catalog # 2354-RP) with a linear range of 0.15 ‑ 5 µg/mL.

• Source

  E. coli-derived Met1-Lys183

• Accession #

• N-terminal Sequence    
  Analysis

  Met1

• Predicted Molecular Mass

  21 kDa

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: IL-36 beta/IL-1F8

Mouse interleukin‑36 beta [IL‑36 beta ; previously IL‑1F8 and also named FIL‑1 eta (eta) and IL‑1H2] is a member of the IL‑1 family of proteins (1 ‑ 3, 6). IL‑1 family members include IL‑1 beta, IL‑1 alpha, IL‑1ra, IL‑18, IL‑36Ra/IL‑1F5, IL‑36 alpha /IL‑1F6, IL‑37/IL‑1F7, IL‑36 gamma /IL‑1F9 and IL‑1F10 (4, 6). All family members show a 12 beta ‑stranded beta ‑trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). Although two alternatively spliced transcript variants for human IL‑36 beta /IL‑1F8 have been described, to date, only one mouse IL‑36 beta /IL‑1F8 isoform is known (3). Mouse IL‑36 beta /IL‑1F8 is synthesized as a 183 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N‑linked glycosylation site(s) (1, 2). It is likely that mouse IL‑36 beta /IL‑1F8, similarly to its human homologue, is actively secreted (1). Mouse IL‑36 beta /IL‑1F8 shares 61% and 74% aa identity with human IL‑36 beta /IL‑1F8 isoform 2 and rat IL‑1F8, respectively. Within the IL‑1 family, IL‑36 beta /IL‑1F8 shares 27% sequence with 33%, 32%, 35%, 39% and 28% aa sequence identity with IL‑1ra, IL‑1 beta, IL‑36Ra/IL‑1F5, IL‑36 alpha /IL‑1F6, IL‑36 gamma /IL‑1F9 and IL‑1F10, respectively. Cells reported to express IL‑36 beta /IL‑1F8 include resting and activated monocytes and B cells (1, 4). The receptor for IL‑36 beta /IL‑1F8 is reported to be a combination of IL‑1 Rrp2 and IL‑1 RAcP (5). Recombinant IL‑36 beta /IL‑1F8, along with IL‑36 alpha /IL‑1F6 and IL‑36 gamma /IL‑1F9, has been shown to activate the pathway involving NF‑ kappa B and MAPK in an IL‑1 Rrp2 dependent manner.

• References:

2. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.

3. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.

4. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.

5. Dunn, E. et al. (2001) Trends Immunol. 22:533.

6. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

7. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.

• Long Name:

  Interleukin 36 beta/Interleukin 1 Family 8

• Entrez Gene IDs:

  27177 (Human); 69677 (Mouse)

• Alternate Names:

  family of interleukin 1-eta; FIL1 eta; FIL1; FIL1-(ETA); FIL1H; FILI-(ETA); IL-1 eta; IL1-ETA; IL1F8 (Canonical product IL-1F8a); IL-1F8 (FIL1-eta); IL1F8; IL-1F8; IL1H2; IL-1H2; IL36 beta; IL-36 beta; IL36B; interleukin 1 family, member 8 (eta); interleukin 1, eta; Interleukin 36, Beta; Interleukin-1 eta; interleukin-1 family member 8; Interleukin-1 homolog 2; Interleukin-1 Superfamily e; Interleukin-36 Beta