详细说明
- Purity>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured in a cell proliferation assay using human pancreatic cancer cells. The ED 50 for this effect is 1-4 μg/mL.
Measured in a cell proliferation assay using EJG bovine adrenal-derived endothelial cells. The ED 50 for this effect is 1-4 µg/mL. - SourceMouse myeloma cell line, NS0-derived Ala20-Phe105
- Accession #
- N-terminal Sequence
AnalysisAla20 - Predicted Molecular Mass9.6 kDa
- SDS-PAGE9.6 kDa, reducing conditions
| 2464-EV/CF | | 2464-EV |
| Formulation Supplied as a 0.2 μm filtered solution in PBS. | Formulation Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | |
| Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Endocrine gland-derived vascular endothelial cell growth factor (EG-VEGF; also called prokineticin-1) is a member of the prokineticin family of secreted proteins whose distinguishing structural motif involves 10 cysteines that form five intrachain disulfide bonds, creating a “colipase fold” (1, 2). The mouse EG‑VEGF precursor is 105 amino acids in length and contains a 19 aa signal sequence plus an 86 aa mature segment (3). There are no potential N‑linked glycosylation sites, and the secreted molecule is a monomer that is approximately 10 kDa in size. The mature region in mouse is 93% and 87% aa identical to the mature regions in rat and human, respectively. Mature mouse prokineticin-2 is 57% aa identical to mouse EG‑VEGF/prokineticin-1. Mouse EG‑VEGF is active on bovine endothelial cells (3). Although there is considerable aa identity between mouse and human EG-VEGF, the expression patterns are very much different. This is attributed to pronounced differences in the promoter region of the two genes. While human EG-VEGF is expressed by steroidogenic glands, mouse EG‑VEGF is expressed in hepatocytes and renal tubular epithelial cells. EG-VEGF binds to and activates two closely related G-protein coupled receptors, EG‑VEGF/PK1‑R1 and EG‑VEGF/PK2‑R2 (4, 5). Mouse EG‑VEGF stimulates proliferation and survival of liver sinusoidal endothelial cells, and, in mouse, EG-VEGF acts to induce monocyte migration and stimulate hematopoiesis (3, 6).
- References:
- LeCouter, J. et al. (2004) Ann. N.Y. Acad. Sci. 1014:50.
- Li, M. et al. (2001) Mol. Pharmacol. 59:692.
- LeCouter, J. et al. (2003) Endocrinology 144:2606.
- Lin, D. et al. (2002) J. Biol. Chem. 277:19276.
- Masuda, Y. et al. (2002) Biochem. Biophys. Res. Commun. 293:396.
- LeCouter, J. et al. (2004) Proc. Natl. Acad. Sci. USA 101:16813.
- Long Name:Endocrine Gland-derived Vascular Endothelial Growth Factor
- Entrez Gene IDs:84432 (Human); 246691 (Mouse); 192205 (Rat)
- Alternate Names:EGVEGF; EG-VEGF; EGVEGFblack mamba toxin-related protein; Endocrine-gland-derived vascular endothelial growth factor; Mambakine; PK1mambakine; PRK1prokineticin-1; PROK1; Prokineticin 1
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