Recombinant Mouse LRP-6 Protein, CF 25 UG

所属行业:生物试剂     有0人感兴趣

Recombinant Mouse LRP-6 Protein, CF 25 UG信息二维码

微信扫码

 
基础投资电议
推荐指数
  • 区域全国
  • 留言暂无
  • 浏览0
  • 更新2024-07-25 20:43
  • 到期长期有效






更多>我还有产品
3-Amino-5-methoxycarbonylphenylboronic acid, pinacol ester  1 g 2,3-Dichloro-6-(trifluoromethyl)benzyl bromide  1 g 1-(4-Fluorophenyl)-5-methoxycarbonyl-2(1H)-pyridinone  1 g N,N-Diethyl-cyclohexane-1,4-diamine  1 g N-Methyl-DL-leucine hydrochloride  5 g N-(2-Chloroethyl) 3-boronobenzamide  5 g

产品介绍

    基本参数

    详细说明

    • Purity
      >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
    • Endotoxin Level
      <0.1 EU per 1 μg of the protein by the LAL method.
    • Activity
      Measured by its binding ability in a functional ELISA. rmLRP-6 binds rhDkk-1, biotin with an apparent K D <15 nM. Biotinylated rmWnt-3a immobilized on a streptavidin-coated plate at 1 µg/mL can bind rmLRP-6 with a linear range of 0.2-10 µg/mL.
    • Source
      Mouse myeloma cell line, NS0-derived Ala20-Pro1368, with a C-terminal 6-His tag
    • Accession #
    • N-terminal Sequence
      Analysis
      Ala20
    • Predicted Molecular Mass
      152.6 kDa
    • SDS-PAGE
      170 kDa, reducing conditions
    2960-LR
     
    Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
    Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
    Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
    Background: LRP-6

    The low-density lipoprotein (LDL) receptor-related protein 5 (LRP-5) and LRP-6 constitute a distinct subgroup of the LDL receptor family (1). Both LRP-5 and LRP-6 are type I transmembrane proteins that function as Wnt co-receptors with Frizzled proteins (FZD) (2, 3, 4). The mouse LRP-6 cDNA encodes a 1613 amino acid (aa) residue precursor including a 19 aa signal sequence, 1351 aa extracellular domain (ECD), a 23 aa transmembrane (TM) segment, and a 20 aa cytoplasmic domain (5). The ECD contains 20 YWTD motif-containing LDLR-B domains, fourEGF-like repeats, and three cysteine-rich LDLR-A repeats. The ECD of mouse LRP-6 shares 71% aa sequence identity with the ECD of mouse LRP-5 and 98% aa sequence identity with the ECD of human, rat, and canine LRP-6. The intracellular region of LRP-6 contains repeated PPPSP motifs. When the Ser/Thr in these motifs are phosphorylated, LRP-6 can interact with Axin and propagate canonical Wnt signal transduction (6, 7). LRP-6 forms inactive homodimers via its YWTD-EGF domains (8). Wnt binding to FZD and subsequent association with LRP-6 lead to activating conformational changes in LRP-6 cytoplasmic domains (8). LRP-6 can also interact directly with the Dickkopf (Dkk), sclerostin, and Wise proteins which are modulators of Wnt signaling (9 - 14). Formation of a ternary complex of LRP-6, Dkk-1, and Kremen triggers the internalization of the complex and removal LRP-6 from the cell surface. LRP-5 and LRP-6 share overlapping functions in diverse embryonic developmental processes (15).

    • References:
      1. Schneider, W.J. and J. Nimpf (2003) Cell Mol Life Sci. 60:892.
      2. He, X. et al. (2004) Development 131:1663.
      3. Seto, E.S. and H.J. Bellen (2004) Trends Cell Biol. 14:45. 
      4. Pinson, K.I. et al. (2000) Nature 407:535. 
      5. Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248:879.
      6. Tamai, K. et al. (2000) Nature 407:530. 
      7. Tamai, K. et al. (2004) Mol. Cell 13:149. 
      8. Liu, G. et al. (2003) Mol. Cell. Biol. 23:5825.
      9. Mao, B. et al. (2001) Nature 411:321.
      10. Semenov, M.V. et al. (2001) Curr. Biol. 11:951.
      11. Bafico, A. et al. (2001) Nat. Cell Biol. 3:683.
      12. Li, X. et al. (2005) J. Biol. Chem. 280:19883.
      13. Semenov, M. et al. (2005) J. Biol. Chem. 280:26770.
      14. Itasaki, N. et al. (2003) Development 130:4295.
      15. Kelly, O. et al. (2004) Development 131:2803.
    • Long Name:
      LDL Receptor-related Protein 6
    • Entrez Gene IDs:
      4040 (Human); 16974 (Mouse)
    • Alternate Names:
      ADCAD2; FLJ90062; FLJ90421; low density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP6; LRP-6
    举报收藏 0评论 0
 
热门项目排行榜快速发布信息
14-(2-碘代乙基)辛基苯 9
2高温波导测试
3格列美脲 98%+ RG  5g
44-溴-3,5-二氯三氟甲苯
5二次谐波
6远红外云试剂
生物试剂排行榜快速发布信息
1Recombinant Human LILR
2Recombinant Human CD47
3Recombinant Human FGF
4203mm具砂板闪式层析柱,
5pUCm-T 载体 5UG
65ml Protein A40 Plus亲
最新加入项目快速发布信息
1质粒大量抽提试剂盒(通
2实验试剂
3产品标题产品标题产品标
4实验试剂
53-Amino-5-methoxycarbo
6N,N-Diethyl-cyclohexan

猜你喜欢产品快速发布信息

相关栏目

还没找到您需要的生物试剂产品?立即发布您的求购意向,让生物试剂公司主动与您联系!

立即发布项目意向

免责声明

本网页所展示的有关【Recombinant Mouse LRP-6 Protein, CF 25 UG_生物试剂_云试剂自营四店】的信息/图片/参数等由云试剂的会员【云试剂自营四店】提供,由云试剂会员【云试剂自营四店】自行对信息/图片/参数等的真实性、准确性和合法性负责,本平台(本网站)仅提供展示服务,请谨慎交易,因交易而产生的法律关系及法律纠纷由您自行协商解决,本平台(本网站)对此不承担任何责任。您在本网页可以浏览【Recombinant Mouse LRP-6 Protein, CF 25 UG_生物试剂_云试剂自营四店】有关的信息/图片/价格等及提供【Recombinant Mouse LRP-6 Protein, CF 25 UG_生物试剂_云试剂自营四店】的商家公司简介、联系方式等信息。

联系方式

在您的合法权益受到侵害时,欢迎您向www@yunshiji.cn邮箱发送邮件,或者进入《网站意见反馈》了解投诉处理流程,我们将竭诚为您服务,感谢您对云试剂的关注与支持!

按排行字母分类找加盟项目:

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z