详细说明
- Purity>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
- Endotoxin Level<1.0 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 2.5‑5.0 µg/mL of Recombinant Mouse Semaphorin 3E Fc Chimera causes >50% growth cone collapse in the presence of 10 ng/mL of Recombinant Human beta ‑NGF (Catalog # ).
- SourceSpodoptera frugiperda, Sf 21 (baculovirus)-derived
Mouse Semaphorin 3E
Asn27-Phe766 (Arg557Ala and Arg560Ala)
Accession # P70275IEGRMD Human IgG1
(Pro100-Lys330)N-terminus C-terminus - Accession #
- N-terminal Sequence
AnalysisAsn27 - Structure / FormDisulfide-linked homodimer
- Predicted Molecular Mass112 kDa (monomer)
- SDS-PAGE110-120 kDa, reducing conditions
3238-S3 | | |
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Tween® 20. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
- References:
- Oh, W.J. and C. Gu (2013) Semin. Cell Dev. Biol. 24:156.
- Adams, R. H. et al. (1997) EMBO J. 16:6077.
- Christensen, C. et al. (2005) Cancer Res. 65:6167.
- Casazza, A. et al. (2010) J. Clin. Invest. 120:2684.
- Bellon, A. et al. (2010) Neuron 66:205.
- Chauvet, S. et al. (2007) Neuron 56:807.
- Bribian, A. et al. (2014) Nat. Commun. 5:4265.
- Ding, J.B. et al. (2011) Nat. Neurosci. 15:215.
- Pecho-Vrieseling, E. et al. (2009) Nature 459:842.
- Cariboni, A. et al. (2015) J. Clin. Invest. 125:2413.
- Gu, C. et al. (2005) Science 307:265.
- Gitler, A. D. et al. (2004) Developmental Cell 7:107.
- Wanschel, A. et al. (2013) Arterioscler. Thromb. Vasc. Biol. 33:886.
- Shimizu, I. et al. (2013) Cell Metab. 18:491.
- Yagai, T. et al. (2014) Am. J. Pathol. 184:2250.
- Movassagh, H. et al. (2014) J. Allergy Clin. Immunol. 133:560.
- Choi, Y.I. et al. (2014) Proc. Natl. Acad. Sci. USA 111:379.
- Entrez Gene IDs:9723 (Human); 20349 (Mouse)
- Alternate Names:(semaphorin) 3E; coll-5; KIAA0331M-SEMAH; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema3E; SEMAH; SEMAHM-SemaK; Semaphorin 3E; semaphorin-3E