详细说明
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Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
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Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
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Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. This protein is able to significantly enhance neurite outgrowth in the range of 60 ng/3 μL drop‑300 ng/3 μL drop.
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Source
Mouse myeloma cell line, NS0-derived Gly22-Asp291 with a C-terminal 6-His tag
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Accession #
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N-terminal Sequence
Analysis
Gly22
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Predicted Molecular Mass
30 kDa
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SDS-PAGE
33 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3475-MN | | 3475-MN/CF |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | | Formulation Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. | | Reconstitution Reconstitute at 100 μg/mL in PBS. |
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
| | Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Meteorin
Meteorin, named after meteor because this protein can transform glial cells into cells with an elongated tail, is a 30 kDa member of the Meteorin family (1). The secreted protein is also known as glial cell differentiation factor and hypoxia/reoxygenation regulatory factor. Mouse Meteorin is synthesized as a 291 amino acid (aa) precursor with a 21 aa signal sequence and a 270 aa mature chain (SwissProt # Q8C1Q4). Splicing variants produce two isoforms. Isoform 2 is missing residues 1 - 164 found in isomer 1, and has a two aa substitution for aa 165 - 166. Mouse Meteorin is 81% aa identical to human Meteorin (2). Meteorin is expressed in the central nervous system both during development and in adult mice (2). During development, Meteorin mRNA appears both in the central and peripheral nervous systems with the most prominent levels in neural progenitors, glial progenitors, and cells of the astrocyte lineage. In the adult mouse, Meteorin is detected only in the brain (2). Within the brain, the most prominent expression is found in the cerebellum where it is expressed by glial cells interspersed between Purkinje neurons (2). Meteorin is also detectable in several discrete neuronal populations, such as the superior colliculus, the ocular motor nucleus, the raphe and pontine nuclei, and various thalamic nuclei (2).Functionally, Meteorin plays important roles in both glial cell differentiation and axonal network formation during neurogenesis (1). In addition, research shows that when Meteorin is expressed and secreted by perivascular astrocytes, it upregulates thrombospondin-1/-2 to attenuate angiogenesis in the surrounding endothelial cells and to promote vascular maturation (3).