• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to induce IFN-gamma secretion by NK‑92 human natural killer lymphoma cells. Brandt, C.S.     et al. (2009) J. Exp. Med.     206:1495. The ED    ₅₀ for this effect is 0.6-3 μg/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Human B7-H6 (Met1 - Ser262) Accession # NP_001189368	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Asp25

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  53.3 kDa (monomer)

• SDS-PAGE

  75-85 kDa, reducing conditions

Data Images

Background: B7-H6

B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins (1, 2). Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53% - 56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis (7).

• References:

1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.

2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.

3. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.

4. Li, Y. et al. (2011) J. Exp. Med. 208:703.

5. Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. 108:6223.

6. Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.

7. Byrd, A. et al. (2007) PLoS ONE 2:e1339.

• Long Name:

  B7 Homolog 6

• Entrez Gene IDs:

  374383 (Human); 102121659 (Cynomolgus Monkey)

• Alternate Names:

  B7 homolog 6; B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1