详细说明
- Purity>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED 50 for this effect is typically 2-10 μg/mL.
- SourceHuman embryonic kidney cell, HEK293-derived
Human B7-H7/HHLA2
(Ile23-Asn344)
Accession # Q9UM44IEGRMD Human IgG1
(Pro100-Lys330)N-terminus C-terminus - Accession #
- N-terminal Sequence
AnalysisIle23 - Structure / FormDisulfide-linked homodimer
- Predicted Molecular Mass64 kDa (monomer)
- SDS-PAGE90-100 kDa, reducing conditions
| 8084-B7 | | |
| Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
| Reconstitution Reconstitute at 100 μg/mL in PBS. | ||
| Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: B7-H7/HHLA2
B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins (1, 2). Mature human B7-H7 consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain (3-5). B7‑H7 is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). Soluble B7-H7 inhibits the proliferation of activated CD4 + and CD8 + T cells and their production of IFN-gamma, TNF-alpha, IL-5, IL-10, IL-13, IL-17A, and IL-22 (5).
- References:
- Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
- Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
- Mager, D.L. et al. (1999) Genomics 59:255.
- Flajnik, M.M. et al. (2012) Immunogenetics 64:571.
- Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.
- Long Name:B7 Homolog 7
- Entrez Gene IDs:11148 (Human)
- Alternate Names:B7H7; B7-H7; HERV-H LTR-associating 2; HERV-H LTR-associating protein 2; HHLA2; Human endogenous retrovirus-H long terminal repeat-associating protein 2
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