详细说明
- Purity>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED 50 for this effect is 0.6-3 μg/mL.
- SourceHuman embryonic kidney cell, HEK293-derived
Cynomolgus Monkey VISTA/B7-H5
(Phe33-Ala194)
Accession # XP_005565644IEGRMD Human IgG1
(Pro100-Lys330)N-terminus C-terminus - Accession #
- N-terminal Sequence
AnalysisPhe33 - Structure / FormDisulfide-linked homodimer
- Predicted Molecular Mass45 kDa
- SDS-PAGE62-71 kDa, reducing conditions
9408-B7 | | |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 200 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Bioactivity | Recombinant Cynomolgus VISTA/B7-H5 Fc Chimera (Catalog #9408-B7) inhibits anti-CD3antibody-induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.6-3 µg/mL. |
- References:
- Flajnik, M.F. et al. (2012) Immunogenetics 64:571.
- Wilcox, R.A. et al. (2012) Eur. J. Haematol. 88:465.
- Sakr, M.A. et al. (2010) Cancer Sci. 101:2368.
- Aloia, L. et al. (2010) J. Biol. Chem.285:7776.
- Parisi, S. et al. (2012) FASEB J. 26:3957.
- Wang, L. et al. (2011) J. Exp. Med. 208:577.
- Flies, D.B. et al (2014) J Clin Invest. 124:1966.
- Lines, J.L. et al. (2014) Cancer Res. 74:1924.
- Nowak, E.C. et al. (2017) Immunol Rev. 276:66.
- Entrez Gene IDs:64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)
- Alternate Names:4632428N05Rik; B7H5; B7-H5; C10orf54; chromosome 10 open reading frame 54; Dies1; Gi24; PD1H; PD-1H; platelet receptor Gi24; PP2135; SISP1; stress induced secreted protein 1; VISTA