详细说明
- Purity>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its binding ability in a functional ELISA. When 15 ng/mL of biotinylated recombinant human Insulin is added to serially diluted Recombinant Human Insulin R/CD220, the concentration of Recombinant Human Insulin R/CD220 that produces 50% of the optimal binding response is 0.03‑0.15 μg/mL.
- SourceMouse myeloma cell line, NS0-derived His28-Arg750 ( alpha subunit) & Ser751-Lys944 with a C-terminal 10-His tag ( beta subunit)
- Accession #
- N-terminal Sequence
AnalysisHis28 ( alpha subunit) & Ser751 ( beta subunit)
- Structure / FormTetramer; disulfide-linked homodimer of disulfide-linked heterodimers ( alpha & beta )
- Predicted Molecular Mass82.9 kDa ( alpha subunit), 22.9 kDa ( beta subunit)
- SDS-PAGE122-135 kDa and 33-43 kDa, reducing conditions
| 1544-IR | | 1544-IR/CF |
| Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. | Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | |
| Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | |
| Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
The Insulin Receptor (gene name INSR, designated CD220) is a type I transmembrane glycoprotein in the Insulin/IGF Receptor family of receptor tyrosine kinases that share structural similarity and overlapping intracellular signaling events (1-3). The 1370 amino acid (aa) human Insulin R preproprotein (A isoform) is processed by proteolysis to remove the signal peptide and produce an extracellular alpha portion (aa 28-750), and an extracellular/transmembrane/cytoplasmic beta subunit (aa 751-1370) (4). The extracellular domain (ECD) contains two homologous globular domains separated by a cysteine-rich domain and followed by three fibronectin type III domains. The intracellular region contains insulin-receptor substrate (IRS) docking sites, the kinase domain, and a phosphotyrosine-containing linker region. The human Insulin R ECD shares 96% aa sequence identity with mouse, rat, equine and canine Insulin R. Two alternatively spliced Insulin R isoforms differ by the absence
(IR-A) or presence (IR-B) of 12 aa between aa 743-744 in the alpha subunit (4). IR-A expression is highest in fetal tissues and cancer cells, while IR-B is concentrated in adult differentiated cells (2-5). IR-A and IR-B may homodimerize, or heterodimerize with the IGF-I receptor (1, 3, 4). All receptor combinations bind insulin, IGF-I or IGF-II, but with differing affinities; for example, IR-A has considerably higher affinity for IGF-II as compared to IR-B (2-5). This system allows fine tuning of signaling pathways according to the concentrations of insulin, IGF-I and IGF-II, and expression of receptor subunits on the cell surface (2, 3). Insulin R signaling regulates glucose uptake and metabolism, but also contributes to cell growth, differentiation and apoptosis (2, 3, 5, 6). Mutations in the Insulin R gene have been linked severe insulin resistance (type A and Rabson-Mendenhall syndrome) that may include type II diabetes mellitus and, rarely, leprechaunism (Donohue syndrome) that also includes growth delays and endocrine system abnormalities (1, 7). The R&D Systems human Insulin R consists of the entire ECD of the IR-A isoform.
- References:
- Nakae, J. et al. (2001) Endoc. Rev. 22:818.
- Sciacca, L. et al. (2003) Endocrinology 144:2650.
- Belfiore, A. et al. (2009) Endocrine Rev. 30:586.
- Lawrence, M.C. et al. (2007) Curr. Opin. Struct. Biol. 17:699.
- Sacco, A. et al. (2009) Endocrinology 150:3594.
- Kitamura, T. et al. (2004) J. Clin. Invest. 113:209.
- Musso, C. et al. (2004) Medicine (Baltimore) 83:209.
- Long Name:Insulin Receptor
- Entrez Gene IDs:3643 (Human); 16337 (Mouse)
- Alternate Names:CD 220; CD220 antigen; CD220; EC 2.7.10; EC 2.7.10.1; HHF5; INSR; Insulin R; insulin receptor; InsulinR; IR
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