详细说明
- Purity>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to inhibit LIF-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED 50 for this effect is typically 1-6 μg/mL in the presence of 0.3 ng/mL of recombinant human LIF.
- SourceHuman embryonic kidney cell, HEK293-derived Met1-Ser833
- Accession #
- N-terminal Sequence
AnalysisGln45 predicted: No result obtained, sequencing might be blocked - Predicted Molecular Mass89.4 kDa
- SDS-PAGE110-140 kDa, reducing conditions
7487-LR | | |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 300 μg/mL in PBS. | ||
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
- References:
- Muller-Newen, G. (2003) Science STKE 2003:pe40.
- Gearing, D.P. et al. (1991) EMBO J. 10:2839.
- Layton, M.J. et al. (1992) Proc. Natl. Acad. Sci. 89:8616.
- Gearing, D.P. et al. (1992) Science 255:1434.
- Giese, B. et al. (2005) J. Cell Sci. 118:5129.
- Ip, N.Y. et al. (1992) Cell 69:1121.
- Davis, S. et al. (1993) Science 260:1805.
- Metcalf, D (2003) Stem Cells 21:5.
- Kubota, Y. et al. (2008) J. Clin. Invest. 118:2393.
- Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
- Stewart, C.L. et al. (1992) Nature 359:76.
- Cheng, J.-G. et al. (2001) Proc. Natl. Acad. Sci. 98:8680.
- Long Name:Leukemia Inhibitory Factor Receptor alpha
- Entrez Gene IDs:3977 (Human); 16880 (Mouse); 81680 (Rat)
- Alternate Names:CD118 antigen; CD118; FLJ98106; FLJ99923; leukemia inhibitory factor receptor alpha; leukemia inhibitory factor receptor; LIF R alpha; LIF R beta; LIF receptor; LIFR; LIF-R; LIFRa; SJS2; STWS; SWS