详细说明
本产品含热启动的TransTaq® HiFi DNA Polymerase,dNTPs和优化的反应缓冲液,浓度为2×。DNA扩增时,只需加入模板、引物和水,使SuperMix溶液的浓度为1×即可进行反应。扩增产物3'端带“A”碱基,可直接克隆于pEASY®-T载体中。TransTaq HiFi PCR SuperMix I 适用于基因组DNA的扩增 (≤ 15 kb)。
• 减少PCR扩增操作时间。
• 避免因多步操作带来的污染。
• 保真性是EasyTaq® DNA Polymerase的18倍。
Xu R , Qin R , Li H , et al. Generation of targeted mutant rice using a CRISPR-Cpf1 system[J]. Plant Biotechnology Journal, 2017 Jun, 15(6):713-717.
Che L H , Zhang S Q , Li Y , et al. Genome-wide survey of nuclear protein-coding markers for beetle phylogenetics and their application in resolving both deep and shallow-level divergences[J]. Molecular Ecology Resources, 2017, 17(6):1342-1358.
Liu T , Sun L , Jiang B , et al. Lineage conversion of mouse fibroblasts to pancreatic α-cells[J]. Experimental & Molecular Medicine, 2017, 49(6):e350.
Hao J , Zhang C , Liang T , et al. rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner[J]. Cellular and Molecular Immunology, 2014, 11(2):206-214.
Feng Y J , Liu Q F , Chen M Y , et al. Parallel tagged amplicon sequencing of relatively long PCR products using the Illumina HiSeq platform and transcriptome assembly[J]. Molecular Ecology Resources, 2016, 16(1):91-102.