详细说明
规格
| Reverse Transcriptase: | RevertAid Reverse Transcriptase |
|---|---|
| Downstream Application: | Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR) |
| Reaction Format: | Separate Components |
| Sample Type (General): | RNA |
| Product Size: | 100 reactions |
| Optimal Reaction Temperature: | 42° C |
内容及储存
Store at -20°C.
描述
Thermo Scientific RevertAid第一链cDNA合成试剂盒包含了足够进行100次x20 µL反应的试剂。本品是以RNA为模板高效合成第一链cDNA的完整系统，可合成长达13kb的cDNA。本品含有RiboLock RNase抑制剂，能够有效保护RNA模板不受降解。
Thermo Scientific RevertAid第一链cDNA合成试剂盒配套提供oligo(dT)18引物和随机六聚体引物。oligo(dT)18引物可以选择性地与mRNA中的poly (A)尾结合。随机六聚体引物无需poly (A)尾，因此可转录mRNA 5’-端区域，也可以无poly (A)尾的RNA (如micoRNA)为模板合成cDNA。该试剂盒也可使用基因特异性引物。
特点：
• 可以高效合成长达13kb的全长第一链cDNA
• 最佳活性温度为42°C
• 完全一提供RT反应所需的所有组分
应用
• 第一链cDNA合成，作为RT-PCR和实时RT-qPCR的模板
• 构建全长cDNA文库
• 反义RNA合成
Full-length cDNA synthesis using the RevertAid First Strand cDNA Synthesis Kit
1 µg of total mouse heart RNA was used in a reverse transcription reaction using oligo(dT)18 primer. The synthesized cDNA was used as a template in subsequent PCR reactions with Taq DNA Polymerase. The ability to amplify the terminal 2024 bp fragment indicates that the entire 13.8 kb RNA sequence was reverse-transcribed.
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